Standard Curve (Sample)
Porcine a1-microglobulin ELISA Kit | A1-MG elisa kit
Porcine a1-microglobulin ELISA Kit
Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Porcine
Synonyms
a1-microglobulin; N/A; Porcine a1-microglobulin ELISA Kit; A1-MG elisa kit
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Detection Range
50-1000ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This A1-MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine A1-MG. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for A1-MG elisa kit
Principle of the Assay: A1-MG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for A1-MG. Standards or samples are then added to the microtiter plate wells and A1-MG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of A1-MG present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for A1-MG are added to each well to "sandwich" the A1-MG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain A1-MG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A1-MG concentration in each sample is interpolated from this standard curve.
Product Categories/Family for A1-MG elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
38,999 Da
NCBI Official Full Name
alpha-1-microglobulin, partial
NCBI Official Synonym Full Names
alpha-1-microglobulin/bikunin precursor
NCBI Official Symbol
AMBP
NCBI Official Synonym Symbols
A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
NCBI Protein Information
protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge
UniProt Protein Name
Protein AMBP
UniProt Gene Name
AMBP
UniProt Synonym Gene Names
HCP; ITIL; Protein HC; ITI-LC
UniProt Entry Name
AMBP_HUMAN
Similar Products
Product Notes
The Porcine A1-MG ambp (Catalog #AAA89799) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA89799 ELISA Kit recognizes Porcine A1-MG. It is sometimes possible for the material contained within the vial of "a1-microglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.