Rat Amyloid Beta Peptide 1-42 (Abeta 1-42) ELISA Kit | Abeta1-42 elisa kit

Rat Amyloid Beta Peptide 1-42 (Abeta 1-42) ELISA Kit

Cat. Num. AAA330231

• Reactivity: Rat
• Synonyms: Amyloid Beta Peptide 1-42 (Abeta 1-42); N/A; Rat Amyloid Beta Peptide 1-42 (Abeta 1-42) ELISA Kit; Abeta1-42 elisa kit

• Reactivity: Rat
• Specificity: Rat Amyloid Beta Peptide 1-42 (Abeta1-42) ELISA Kit has high sensitivity and excellent specificity for detection of Rat Abeta1-42. No significant cross-reactivity or interference between Rat Abeta1-42 and analogues was observed.
• Assay Type: Sandwich
• Samples: Rat serum, cell culture supernates and other biological fluids
• Detection Range: 3 ug/L - 48 ug/L
• Sensitivity: < 0.1 ug/L

• Intra-assay Precision: Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
• Inter-assay Precision: Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for Abeta1-42 elisa kit

Background: Alzheimer's disease (AD) is the most common form of dementia and is a progressive neurodegenerative disorder of insidious onset that causes gradual loss of memory and cognitive function. One of the hallmarks of Alzheimer's disease is the self-aggregation of the amyloid beta peptide (Abeta) in extracellular amyloid fibrils. Abeta is a peptide composed of 40 to 42 (43) amino acids. Among the different forms of Abeta, the 42-residue fragment (Abeta1-42) readily self-associates and forms nucleation centers from where fibrils can quickly grow. The strong tendency of Abeta1-42 to aggregate is one of the reasons for the scarcity of data on its fibril formation process. Abeta1-42 is the major component of senile plaques and considered as the most crucial factor in AD pathogenesis.

Principle of the Assay: Rat Amyloid Beta Peptide 1-42 (Abeta1-42) ELISA Kit employs a two-site sandwich ELISA to quantitate Abeta1-42 in samples. An antibody specific for Abeta1-42 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Abeta1-42 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat Abeta1-42 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Abeta1-42 bound in the initial step. The color development is stopped and the intensity of the color is measured.