Human actin, alpha 2, smooth muscle, aorta ELISA Kit | ACTA2 elisa kit

Human alpha-Smooth muscle actin, alpha-SMA ELISA Kit

Cat. Num. AAA15183

• Gene Names: ACTA2; AAT6; ACTSA; MYMY5
• Reactivity: Human
• Synonyms: actin, alpha 2, smooth muscle, aorta; N/A; Human alpha-Smooth muscle actin, alpha-SMA ELISA Kit; Human alpha-Smooth muscle actin; alpha-SMA ELISA Kit; AAT6; ACTSA; alpha 2 actin; alpha-cardiac actin; growth-inhibiting gene 46; actin; alpha 2; smooth muscle; aorta; ACTA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human alpha-SMA. No significant cross-reactivity or interference between human alpha-SMA and analogues was observed.
• Sequence Length: 377
• Samples: Serum, tissue homogenates, urine
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 ng/ml -200 ng/ml
• Sensitivity: Typically less than 0.78 ng/ml.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for ACTA2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for alpha-SMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any alpha-SMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for alpha-SMA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of alpha-SMA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 213688375
• NCBI GeneID: 59
• NCBI Accession #: NP_001135417.1
• NCBI GenBank Nucleotide #: NM_001141945.1
• UniProt Accession #: P62736; P03996; P04108; Q6FI19; B2R8A4
• Molecular Weight: 42,009 Da
• NCBI Official Full Name: actin, aortic smooth muscle
• NCBI Official Synonym Full Names: actin, alpha 2, smooth muscle, aorta
• NCBI Official Symbol: ACTA2
• NCBI Official Synonym Symbols: AAT6; ACTSA; MYMY5
• NCBI Protein Information: actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
• UniProt Protein Name: Actin, aortic smooth muscle
• UniProt Gene Name: ACTA2
• UniProt Synonym Gene Names: ACTSA; ACTVS; GIG46
• UniProt Entry Name: ACTA_HUMAN