WB (Western Blot)
(Western Blot; Sample: Lane1: human Placenta lysate; Lane2: Porcine Heart lysate; Lane3: Mouse Heart lysate; Lane4: Mouse Skeletal muscle lysatePrimary Ab: 0.01 ug/ml Mouse Anti-ulti-species ACTa2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody | anti-ACTa2 antibody
Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Dog, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
Actin Alpha 2, Smooth Muscle (ACTa2), Antibody; Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2); Actin Alpha 2, Smooth Muscle; ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein; anti-ACTa2 antibody
Host
Mouse
Reactivity
Human, Dog, Pig
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
E101
Specificity
The antibody is a mouse monoclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Ile167~Phe377
Applicable Applications for anti-ACTa2 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot; Sample: Lane1: human Placenta lysate; Lane2: Porcine Heart lysate; Lane3: Mouse Heart lysate; Lane4: Mouse Skeletal muscle lysatePrimary Ab: 0.01 ug/ml Mouse Anti-ulti-species ACTa2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: human Placenta lysate; Lane2: Porcine Heart lysate; Lane3: Mouse Heart lysate; Lane4: Mouse Skeletal muscle lysatePrimary Ab: 0.01 ug/ml Mouse Anti-ulti-species ACTa2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Canine Heart lysate Primary Ab: 0.001ug/ml Mouse Anti-ulti-species ACTa2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Canine Heart lysate Primary Ab: 0.001ug/ml Mouse Anti-ulti-species ACTa2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-ACTa2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, alpha 2, smooth muscle, aorta, isoform CRA_a
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
NCBI Protein Information
actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_HUMAN
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Product Notes
The ACTa2 acta2 (Catalog #AAA152734) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ile167~Phe377. The Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) reacts with Human, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Actin Alpha 2, Smooth Muscle (ACTa2) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ACTa2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin Alpha 2, Smooth Muscle (ACTa2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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