SDS-PAGE
DAPK1 / DAP Kinase 1 Active Protein | DAPK1 active protein
Recombinant Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST tag)
Gene Names
DAPK1; DAPK
Purity
(78.5 + 12.2%) as determined by SDS-PAGE
Synonyms
DAPK1 / DAP Kinase 1; N/A; Recombinant Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST tag); RP11-104M22.1, MGC126323, MGC126324; DAPK1 active protein
Purity/Purification
(78.5 + 12.2%) as determined by SDS-PAGE
Form/Format
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
Concentration
0.3 mg/mL measured by UV-Vis (varies by lot)
Sequence Length
1430
Source
Human
Expression Host
Baculovirus-insect cells
Protein Construction
A DNA sequence encoding the N-terminal segment of human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-Activity
The specific was determined to be 20 nmo/min/mg using synthetic R11-S6-Peptid (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
Predicted N-terminal
Met
Moleclar Mass
The recombinant human DAPK1 (aa 1-363)/GST chimera consists of 600 amino acids and has a calculated molecular mass of 69.4 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Component
Total Volume is 67 uL
Preparation and Storage
Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliqouted and be used as soon as possible.
Avoid repeated freeze-thaw cycles.
Stability: Samples are stable for up to twelve months from date of receipt at -70 °.C
Avoid repeated freeze-thaw cycles.
Stability: Samples are stable for up to twelve months from date of receipt at -70 °.C
SDS-PAGE
SDS-PAGE
Related Product Information for DAPK1 active protein
Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains ten ANK repeats, one death domain and one protein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype , inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 (DAPK1) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia (CML).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
death-associated protein kinase 1
NCBI Official Synonym Full Names
death associated protein kinase 1
NCBI Official Symbol
DAPK1
NCBI Official Synonym Symbols
DAPK
NCBI Protein Information
death-associated protein kinase 1
UniProt Protein Name
Death-associated protein kinase 1
UniProt Gene Name
DAPK1
UniProt Synonym Gene Names
DAPK; DAP kinase 1
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Product Notes
The DAPK1 dapk1 (Catalog #AAA173507) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DAPK1 / DAP Kinase 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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