Purity Info
(The purity was determined to be >80% by densitometry. Approx. MW 64kDa.)
PDE1A active protein
PDE1A, Active
Gene Names
PDE1A; HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A
Purity
>80%
Synonyms
PDE1A; N/A; PDE1A, Active; PDE1A active protein
Isotype
Sf9 cells
Specificity
Full length protein expressed in Sf9 cells
Purity/Purification
>80%
Form/Format
Recombinant protein stored in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.1 ug/ul (varies by lot)
Sequence Length
4918
Specific Activity
10,500 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on dry ice. Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity Info
(The purity was determined to be >80% by densitometry. Approx. MW 64kDa.)
Purity Info
(The purity was determined to be >80% by densitometry. Approx. MW 64kDa.)
Specific Activity
(The specific activity of PDE1A was determined to be 10,500 nmol/min/mg as per activity assay protocol)
Specific Activity
(The specific activity of PDE1A was determined to be 10,500 nmol/min/mg as per activity assay protocol)
Related Product Information for PDE1A active protein
Description: Recombinant full-length human PDE1A was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A (1). Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis (2). In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.
Scientific Background: PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A (1). Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis (2). In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.
Product Categories/Family for PDE1A active protein
References
1. Lefi¨vre L, et al: Presence of cyclic nucleotide phosphodiesterases PDE1A, existing as a stable complex with calmodulin, and PDE3A in human spermatozoa. Biol Reprod. 2002 Aug;67 (2):423-30. 2. Nagel D J, et al: Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle cell growth and survival. Circ Res. 2006 Mar 31;98 (6):777-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~64 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 1A (PDE1A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 1A
NCBI Official Symbol
PDE1A
NCBI Official Synonym Symbols
HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A
NCBI Protein Information
calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
UniProt Protein Name
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
UniProt Gene Name
PDE1A
UniProt Synonym Gene Names
Cam-PDE 1A
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Product Notes
The PDE1A pde1a (Catalog #AAA73096) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PDE1A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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