Mouse Phosphodiesterase 1A, Calmodulin Dependent ELISA Kit | PDE1A elisa kit

Mouse Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) (Sandwich ELISA) ELISA Kit

Cat. Num. AAA189990

• Gene Names: Pde1a; AI987702; AW125737
• Reactivity: Mouse
• Synonyms: Phosphodiesterase 1A, Calmodulin Dependent; N/A; Mouse Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) (Sandwich ELISA) ELISA Kit; hCam-1; 61 kDa Cam-PDE; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A; ELISA Kit for Phosphodiesterase 1A, Calmodulin Dependent (PDE1A); PDE1A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A).; No significant cross-reactivity or interference between Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) and analogues was observed. Reactive with Mouse PDE1A / Phosphodiesterase 1A, Calmodulin Dependent
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.124ng/mL

• Detection Method: Colormetric
• Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
• Preparation and Storage: 4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year

Standard Curve (Sample)

(Typical Standard Curve for PDE1A ELISA (Sandwich))

Related Product Information for PDE1A elisa kit

Principle of the Assay||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1A, Calmodulin Dependent (PDE1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PDE1A elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 568915799
• NCBI GeneID: 18573
• NCBI Accession #: XP_006498986.1
• NCBI GenBank Nucleotide #: XM_006498923.3
• UniProt Accession #: Q61481; O35388; E9Q6V1
• Molecular Weight: 60,685 Da
• NCBI Official Synonym Full Names: phosphodiesterase 1A, calmodulin-dependent
• NCBI Official Symbol: Pde1a
• NCBI Official Synonym Symbols: AI987702; AW125737
• NCBI Protein Information: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
• UniProt Protein Name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
• UniProt Gene Name: Pde1a
• UniProt Synonym Gene Names: Cam-PDE 1A