ALK4/ACVR1B Recombinant Protein | ACVR1B recombinant protein

ALK4/ACVR1B Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA257427

• Gene Names: ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4
• Purity: >90 % as determined by SDS-PAGE.
• Synonyms: ALK4/ACVR1B; N/A; ALK4/ACVR1B Protein, Human, Recombinant (ECD, hFc Tag); Human ALK4/ACVR1B Protein (Fc Tag); activin A receptor, type IB; ACTRIB Protein, Human; ACVRLK4 Protein, Human; ALK4 Protein, Human; SKR2 Protein, Human; ACVR1B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 341aa
• Sequence: Met1-Glu126

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Ser24
• Protein Construction: |A DNA sequence encoding the human ACVR1B (NP_004293.1) (Met1-Glu126) was expressed with the Fc region of human IgG1 at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human TDGF1-His (Cat:10908-H08H) at 10ug/mL (100uL/well) can bind ACVR1B-Fc (Cat:10583-H02H). The EC50 of human ACVR1B-Fc (Cat:10583-H02H) is 3-20ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized human TDGF1-His (Cat:10908-H08H) at 10 ug/mL (100 uL/well) can bind ACVR1B-Fc (Cat:10583-H02H), the EC50 of human ACVR1B-Fc (Cat:10583-H02H) is 3-20 ng/mL.)

Application Data

Related Product Information for ACVR1B recombinant protein

ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.

References

Chen Y, et al. (2005) Developmental analysis of activin-like kinase receptor-4 (ALK4) expression in Xenopus laevis. 232(2): 393-8.J. Massagu. (1998) TGF-SIGNAL TRANSDUCTION. Annual Review of Biochemistry. 67: 753-91.

NCBI and Uniprot Product Information

• NCBI GI #: 4757720
• NCBI GeneID: 91
• NCBI Accession #: NP_004293.1
• NCBI GenBank Nucleotide #: NM_004302.4
• UniProt Accession #: P36896; Q15479; Q15480; Q15481; Q15482; B7Z5L8; B7Z5W5
• Molecular Weight: 56,807 Da
• NCBI Official Full Name: activin receptor type-1B isoform a
• NCBI Official Synonym Full Names: activin A receptor, type IB
• NCBI Official Symbol: ACVR1B
• NCBI Official Synonym Symbols: ALK4; SKR2; ACTRIB; ACVRLK4
• NCBI Protein Information: activin receptor type-1B; activin receptor-like kinase 4; activin A receptor, type II-like kinase 4; serine/threonine-protein kinase receptor R2
• UniProt Protein Name: Activin receptor type-1B
• UniProt Gene Name: ACVR1B
• UniProt Synonym Gene Names: ACVRLK4; ALK4; ACTR-IB; ALK-4; SKR2
• UniProt Entry Name: ACV1B_HUMAN