Standard Curve (Sample)
Adenovirus Hexon ELISA Kit
Adenovirus Hexon ELISA Kit
Reactivity
Adenovirus
Synonyms
Adenovirus Hexon; N/A; Adenovirus Hexon ELISA Kit; Adenovirus Hexon elisa kit
Reactivity
Adenovirus
Detection Method
Colorimetric
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Adenovirus Hexon elisa kit
Background: Adenoviruses are non-enveloped viruses with icosahedral capsids of 90 to 100 nm in size containing a double stranded DNA genome ranging from 26 to 46 kb. Recombinant adenoviruses have been developed as gene delivery vectors for recombinant vaccines and gene therapy applications, including the treatment of metabolic disorders and cancers. Hexon is the largest and most abundant of the structural protein in the icosahedral adenovirus capsid. Each adenoviral particle contains 240 copies of the hexon trimer. The other two major capsid proteins, the penton base and fiber, form the penton complex at each virion vertex. The Adenovirus Hexon ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the adenovirus hexon protein. The ELISA antibodies against adenoviral hexon protein recognizes all 51 serotypes of adenovirus. The kit has a detection sensitivity limit of 156 pg /mL hexon. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and hexon samples.
Principle of the Assay: An anti-adenoviral hexon monoclonal coating antibody is adsorbed onto a microtiter plate. Adenoviral hexon present in the sample or standard binds to the antibodies adsorbed on the plate; an FITC-conjugated anti-hexon polyclonal antibody is added and binds to the hexon antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-hexon polyclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of adenovirus hexon present in the sample. The reaction is terminated by addition of Stop Solution and absorbance is measured at 450 nm. A standard curve is prepared from hexon standard and sample hexon concentration is then determined.
Principle of the Assay: An anti-adenoviral hexon monoclonal coating antibody is adsorbed onto a microtiter plate. Adenoviral hexon present in the sample or standard binds to the antibodies adsorbed on the plate; an FITC-conjugated anti-hexon polyclonal antibody is added and binds to the hexon antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-hexon polyclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of adenovirus hexon present in the sample. The reaction is terminated by addition of Stop Solution and absorbance is measured at 450 nm. A standard curve is prepared from hexon standard and sample hexon concentration is then determined.
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Product Notes
The Adenovirus Adenovirus Hexon (Catalog #AAA44703) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA44703 ELISA Kit recognizes Adenovirus Adenovirus Hexon. It is sometimes possible for the material contained within the vial of "Adenovirus Hexon, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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