ELISA
(ELISA for ADPRH/ARH1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRH/ARH1 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ADPRH/ARH1 recombinant protein
Recombinant ADPRH/ARH1 protein
Synonyms
ADPRH/ARH1; N/A; Recombinant ADPRH/ARH1 protein; ADP-ribosylarginine hydrolase; ADPRH/ARH1 recombinant protein
Host
E coli
Form/Format
Recombinant ADPRH/ARH1 protein is supplied in 25 mM Tris 8.0, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant ADPRH/ARH1 protein that includes full length of the human ADPRH/ARH1 protein (accession number NP_001116.1) was expressed in E coli cells with an C-terminal His tag, The molecular weight of the protein is 40.57kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
ELISA
(ELISA for ADPRH/ARH1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRH/ARH1 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA
(ELISA for ADPRH/ARH1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRH/ARH1 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA
(ELISA for ADPRH/ARH1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRH/ARH1 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA
(ELISA for ADPRH/ARH1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRH/ARH1 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
SDS-PAGE
(Recombinant ADPRH/ARH1 protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 40.57kDa Purity: >90%)
SDS-PAGE
(Recombinant ADPRH/ARH1 protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 40.57kDa Purity: >90%)
Related Product Information for ADPRH/ARH1 recombinant protein
Protein ADP-ribosylation is a highly dynamic post-translational modification. The rapid turnover is achieved, among others, by ADP-(ribosyl)hydrolases (ARHs), an ancient family of enzymes that reverses this modification. Recently ARHs came into focus due to their role as regulators of cellular stresses and tumor suppressors.ADPRH/ARH1 (ADPribosylhydrolase ARH1) as one of ARHs.specifically acts as an arginine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to arginine residues on proteins.
Product Categories/Family for ADPRH/ARH1 recombinant protein
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Product Notes
The ADPRH/ARH1 (Catalog #AAA60375) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ADPRH/ARH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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