Standard Curve (Sample)
Human Adrenergic Receptor, Alpha 1A ELISA Kit | ADRAlpha 1A elisa kit
Human Adrenergic Receptor, Alpha 1A ELISA Kit
Gene Names
ADRA1D; DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
Reactivity
Human
Synonyms
Adrenergic Receptor, Alpha 1A; N/A; Human Adrenergic Receptor, Alpha 1A ELISA Kit; ADRAlpha 1A elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ADRAlpha 1A elisa kit
Intended Uses: This ADPGK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADPGK. This ELISA kit for research use only!
Principle of the Assay: ADPGK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADPGK antibody and an ADPGK-HRP conjugate. The assay sample and buffer are incubated together with ADPGK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPGK concentration since ADPGK from samples and ADPGK-HRP conjugate compete for the anti-ADPGK antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPGK from the sample, fewer sites are left to bind ADPGK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPGK concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ADPGK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADPGK antibody and an ADPGK-HRP conjugate. The assay sample and buffer are incubated together with ADPGK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPGK concentration since ADPGK from samples and ADPGK-HRP conjugate compete for the anti-ADPGK antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPGK from the sample, fewer sites are left to bind ADPGK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPGK concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADRAlpha 1A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,463 Da
NCBI Official Full Name
alpha adrenergic receptor subtype alpha 1a
NCBI Official Synonym Full Names
adrenoceptor alpha 1D
NCBI Official Symbol
ADRA1D
NCBI Official Synonym Symbols
DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
NCBI Protein Information
alpha-1D adrenergic receptor; alpha-1D adrenoceptor; alpha-1D adrenoreceptor; alpha-1A adrenergic receptor; alpha-1D-adrenergic receptor; alpha-adrenergic receptor 1a; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1D-, receptor; adrenergic, alpha -1D-, receptor
UniProt Protein Name
Alpha-1D adrenergic receptor
UniProt Gene Name
ADRA1D
UniProt Synonym Gene Names
ADRA1A; Alpha-1D adrenoceptor
UniProt Entry Name
ADA1D_HUMAN
Similar Products
Product Notes
The Human ADRAlpha 1A adra1d (Catalog #AAA85570) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA85570 ELISA Kit recognizes Human ADRAlpha 1A. It is sometimes possible for the material contained within the vial of "Adrenergic Receptor, Alpha 1A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.