All Products | AAA Biotech

myostatin, ELISA Kit (Cat# AAA18210)

Full Name

Bovine Growth/differentiation factor 8, MSTN ELISA Kit

Gene Names

MSTN; GDF8

Reactivity

Bovine

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of JK cells using anti-TDP-43/TARDBP antibody (AAA19743).Overlay histogram showing JK cells stained with AAA19743 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDP-43/TARDBP Antibody (AAA19743, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of TDP-43/TARDBP using anti-TDP-43/TARDBP antibody (AAA19743) and anti-Beta Tubulin antibody (M01857-3).TDP-43/TARDBP was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse pancrease lysates,Lane 6: mouse Neuro-2a whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP-43/TARDBP antigen affinity purified polyclonal antibody (#AAA19743) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TDP-43/TARDBP at approximately 43 kDa. The expected band size for TDP-43/TARDBP is at 45 kDa.)

TDP-43/TARDBP, Polyclonal Antibody (Cat# AAA19743)

Full Name

Anti-TDP-43/TARDBP Antibody Picoband

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), Recombinant Protein (Cat# AAA20255)

Full Name

Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

Gene Names

Wnt5a; Wnt-5a; 8030457G12Rik

Reactivity

Mouse

Applications

SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Purity

> 95%

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WB (Western Blot) (Western blot analysis of extracts of various cells.)

POLK/DNA Polymerase Kappa, Polyclonal Antibody (Cat# AAA21279)

Full Name

Anti-POLK/DNA Polymerase Kappa Antibody

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Affinity Purified

Pricing

Telomerase reverse transcriptase, ELISA Kit (Cat# AAA23327)

Full Name

Dog Telomerase reverse transcriptase ELISA Kit

Reactivity

Dog

Pricing

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WB (Western Blot) (WB Suggested Anti-SOD2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta)

SOD2, Polyclonal Antibody (Cat# AAA23583)

Full Name

SOD2 antibody - N-terminal region

Gene Names

SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)

DNAJC10, Monoclonal Antibody (Cat# AAA25375)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (HRP)

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged CAMKK2 is ~1ng/ml as a capture antibody.)

CAMKK2, Monoclonal Antibody (Cat# AAA25631)

Full Name

CAMKK2 (Calcium/Calmodulin-dependent Protein Kinase Kinase 2, CaM-kinase Kinase 2, CaM-KK 2, CaMKK 2, Calcium/Calmodulin-dependent Protein Kinase Kinase beta, CaM-kinase Kinase beta, CaM-KK beta, CaMKK beta, CAMKKB, KIAA0787) (PE)

Gene Names

CAMKK2; CAMKK; CAMKKB

Reactivity

Human

Applications

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)

ZNF622, Monoclonal Antibody (Cat# AAA25887)

Full Name

ZNF622 (Zinc Finger Protein 622, Zinc Finger-like Protein 9, ZPR9) (PE)

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (TUBB2A monoclonal antibody (M04), clone 3B2. Western Blot analysis of TUBB2A expression in NIH/3T3.)

TUBB2A, Monoclonal Antibody (Cat# AAA26143)

Full Name

TUBB2A (Tubulin, beta 2A, TUBB, TUBB2, dJ40E16.7) (APC)

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 10 ug/ml])

MAPK3, Monoclonal Antibody (Cat# AAA26399)

Full Name

MAPK3 (Mitogen-Activated Protein Kinase 3, ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3) (FITC)

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

14-3-3 sigma, Polyclonal Antibody (Cat# AAA28959)

Full Name

14-3-3 sigma Polyclonal Antibody

Gene Names

SFN; YWHAS

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

Glutathione (GSH), ELISA Kit (Cat# AAA12832)

Full Name

Human Glutathione (GSH) ELISA Kit

Gene Names

GSTM2; GST4; GSTM; GTHMUS; GSTM2-2

Reactivity

Human

Pricing

Anti-thymocyte globulin, ELISA Kit (Cat# AAA16160)

Full Name

Rabbit Anti-thymocyte globulin ELISA Kit

Reactivity

Rabbit

Pricing

Vitamin K, ELISA Kit (Cat# AAA17184)

Full Name

Rabbit Vitamin K ELISA Kit

Reactivity

Rabbit

Pricing

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APOA1, Antibody (Cat# AAA17952)

Full Name

APOA1 Antibody

Gene Names

APOA1; apo(a)

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA).

Pricing

Thy-1 cell surface antigen, ELISA Kit (Cat# AAA18208)

Full Name

Human Thy-1 membrane glycoprotein, THY1 ELISA Kit

Gene Names

THY1; CD90

Reactivity

Human

Pricing

FGgamma, ELISA Kit (Cat# AAA21534)

Full Name

Human FGgamma (Fibrinogen Gamma chain) ELISA Kit

Reactivity

Human

IL-35, ELISA Kit (Cat# AAA21790)

Full Name

Human IL-35 (Interleukin 35) ELISA Kit

Gene Names

EBI3; IL27B; IL-27B

Reactivity

Human

Pricing

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WB (Western Blot) (Detection of human BMI1 by western blot. Samples: Whole cell lysate (10 ug) from MOLT-4, HeLa, HEK293T, Jurkat, LNCaP, RKO, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

BMI1, Monoclonal Recombinant Antibody (Cat# AAA23838)

Full Name

Rabbit anti-BMI1 Recombinant Monoclonal Antibody [BLR119H]

Gene Names

BMI1; PCGF4; RNF51; FLVI2/BMI1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

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WB (Western Blot) (Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

PGM1, Monoclonal Antibody (Cat# AAA24606)

Full Name

PGM1 (Phosphoglucomutase-1, Glucose Phosphomutase 1) APC

Gene Names

PGM1; CDG1T; GSD14

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged WWP1 is 0.03ng/ml as a capture antibody.)

WWP1, Monoclonal Antibody (Cat# AAA25886)

Full Name

WWP1 (WW Domain-containing Protein 1, Atrophin-1-interacting Protein 5, AIP5, NEDD4-like E3 Ubiquitin-protein Ligase WWP1, TGIF-interacting Ubiquitin Ligase 1, Tiul1, hSDRP1) (PE)

Gene Names

WWP1; AIP5; Tiul1; hSDRP1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

PTPRC/CD45/CD45RA, Recombinant Protein (Cat# AAA28446)

Full Name

Recombinant Human PTPRC/CD45/CD45RA Protein

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Purity

> 95% by SDS-PAGE.

Pricing

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IF (Immunofluorescence) (Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

CD38, Polyclonal Antibody (Cat# AAA28702)

Full Name

CD38 Antibody (C-term)

Gene Names

CD38; T10; ADPRC 1

Reactivity

Human (Predicted Reactivity: Monkey)

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

ERK 1/2, Polyclonal Antibody (Cat# AAA28958)

Full Name

ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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ICC (Immunocytochemistry) (ICC staining CD9 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

CD9, Monoclonal Antibody (Cat# AAA29982)

Full Name

CD9 Antibody

Gene Names

CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CCT4 antibody. Blue: DAPI for nuclear staining.)

CCT4, Polyclonal Antibody (Cat# AAA10783)

Full Name

CCT4 Polyclonal Antibody

Gene Names

CCT4; SRB; Cctd; CCT-DELTA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

Interleukin 37, IL-37, ELISA Kit (Cat# AAA11295)

Full Name

Human Interleukin 37, IL-37 ELISA Kit

Gene Names

IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)

Reactivity

Human

Pricing

enterovirus 71 virus (EV71) antibody (IgM), ELISA Kit (Cat# AAA15391)

Full Name

Human enterovirus 71 virus (EV71) antibody, IgM ELISA Kit

Reactivity

Human

Pricing

lysophosphatidic acid (LPA), ELISA Kit (Cat# AAA15903)

Full Name

Human lysophosphatidic acid, LPA ELISA Kit

Reactivity

Human

Pricing

Vitamin E, ELISA Kit (Cat# AAA16415)

Full Name

Human Vitamin E ELISA Kit

Reactivity

Human

Pricing

Enkephalin, ELISA Kit (Cat# AAA16927)

Full Name

Mouse Enkephalin ELISA Kit

Gene Names

Penk; Enk; Penk1; Penk2; Penk-rs

Reactivity

Mouse

Pricing

Soluble transferrin receptor, ELISA Kit (Cat# AAA17183)

Full Name

Human Soluble transferrin receptor ELISA Kit

Reactivity

Human

Pricing

kelch-like ECH-associated protein 1, ELISA Kit (Cat# AAA18207)

Full Name

Mouse Kelch-like ECH-associated protein 1, KEAP1 ELISA Kit

Gene Names

Keap1; INRF2; mKIAA0132

Reactivity

Mouse

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-LZIC antibody (AAA20000).Overlay histogram showing 293T cells stained with AAA20000 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LZIC Antibody (AAA20000, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of LZIC using anti-LZIC antibody (AAA20000) and anti-Beta Tubulin antibody (M01857-3).LZIC was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LZIC Antibody (AAA20000) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LZIC Antibody (AAA20000) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LZIC Antibody (AAA20000) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human HEL whole cell lysates,Lane 6: human Jurkat whole cell lysates,Lane 7: rat brain tissue lysates,Lane 8: rat thymus tissue lysates,Lane 9: mouse brain tissue lysates,Lane 10: mouse thymus tissue lysates,Lane 11: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LZIC antigen affinity purified polyclonal antibody (#AAA20000) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LZIC at approximately 21 kDa. The expected band size for LZIC is at 21 kDa.)

LZIC, Polyclonal Antibody (Cat# AAA20000)

Full Name

Anti-LZIC Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

Glutathione S-transferase, ELISA Kit (Cat# AAA22560)

Full Name

Porcine glutathione S-transferase (GST) ELISA Kit

Gene Names

GstS1; CG8938; DmelCG8938; DmGst-2; DmGST-2; DmGST2; DmGSTS1; DmGstS1-1; DmGSTS1-1; Est2; GST; GST S1; GST-2; Gst2; GST2; gstD2; GSTII; gsts1; GSTS1; GstS1-1; l(2)04227; l(2)k08805; lincRNA.330; n(2)06253; S1

Reactivity

Porcine

Pricing

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WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in PC-12)

PGRMC2, Monoclonal Antibody (Cat# AAA24608)

Full Name

PGRMC2 (Membrane-associated Progesterone Receptor Component 2, Progesterone Membrane-binding Protein, Steroid Receptor Protein DG6, DG6, PMBP) APC

Gene Names

PGRMC2; DG6; PMBP

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged GTF2H1 is ~1ng/ml as a capture antibody.)

GTF2H1, Monoclonal Antibody (Cat# AAA25120)

Full Name

GTF2H1 (BTF2, General Transcription Factor IIH Subunit 1, Basic Transcription Factor 2 62kD Subunit, General Transcription Factor IIH Polypeptide 1, TFIIH Basal Transcription Factor Complex p62 Subunit) (FITC)

Gene Names

GTF2H1; P62; BTF2; TFB1; TFIIH

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in Raw 264.7.)

RNF2, Monoclonal Antibody (Cat# AAA26400)

Full Name

RNF2 (RING Finger Protein 2, BAP-1, BAP1, DING, HIPI3, Ring1B, Ring2) (FITC)

Gene Names

RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B

Applications

Western Blot (WB)

Purity

Purified

Pricing

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WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)

ACTB, Monoclonal Antibody (Cat# AAA26656)

Full Name

ACTB (Actin, beta, PS1TP5BP1) (PE)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

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Prothrombin, Polyclonal Antibody (Cat# AAA30752)

Full Name

Prothrombin Rabbit Polyclonal Antibody

Gene Names

F2; PT; THPH1; RPRGL2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

Reactive Oxygen Species (ROS), ELISA Kit (Cat# AAA31520)

Full Name

Mouse Reactive Oxygen Species (ROS) ELISA Kit

Reactivity

Mouse

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using AAAS antibody. Blue: DAPI for nuclear staining.)

AAAS, Polyclonal Antibody (Cat# AAA10785)

Full Name

AAAS Polyclonal Antibody

Gene Names

AAAS; AAA; AAASb; GL003; ALADIN; ADRACALA; ADRACALIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Either Herceptin or Samceptin (1 & 2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)

ErbB2/HER2, Monoclonal Recombinant Antibody (Cat# AAA14881)

Full Name

Recombinant anti- human ErbB2/HER2 Antibody (Trastuzumab)

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human

Applications

Functional Assay, Flow Cytometry (FC/FACS)

Purity

>98.0% as determined by SEC-HPLC & SDS-PAGE.

Pricing

Protein S, ELISA Kit (Cat# AAA16417)

Full Name

Mouse Protein S ELISA Kit

Reactivity

Mouse

Pricing

Activating Transcription Factor 3, ELISA Kit (Cat# AAA16673)

Full Name

Human Activating Transcription Factor 3 ELISA Kit

Reactivity

Human

Pricing

Endothelin-1, ELISA Kit (Cat# AAA17441)

Full Name

Human Endothelin-1 ELISA Kit

Gene Names

EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7

Reactivity

Human

Pricing

tumor protein, translationally-controlled 1, ELISA Kit (Cat# AAA18209)

Full Name

Human Translationally-controlled tumor protein, TPT1 ELISA Kit

Gene Names

TPT1; HRF; p02; p23; TCTP

Reactivity

Human

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-METTL18 antibody (AAA20001).Overlay histogram showing A431 cells stained with AAA20001 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL18 Antibody (AAA20001, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of METTL18 using anti-METTL18 antibody (AAA20001) and anti-Beta Tubulin antibody (M01857-3).METTL18 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL18 antigen affinity purified polyclonal antibody (#AAA20001) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for METTL18 at approximately 50 kDa. The expected band size for METTL18 is at 42 kDa.)

METTL18, Polyclonal Antibody (Cat# AAA20001)

Full Name

Anti-METTL18 Antibody Picoband

Gene Names

METTL18; HPM1; AsTP2; C1orf156

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

EPAS-1, Polyclonal Antibody (Cat# AAA29219)

Full Name

EPAS-1 Polyclonal Antibody

Gene Names

EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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