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IHC (Immunohistchemistry) (Anti-Adiponectin antibody, AAA11599, IHC(P)IHC(P): Human Placenta Tissue)

Adiponectin, Polyclonal Antibody (Cat# AAA11599)

Full Name

Anti-Adiponectin antibody

Gene Names

ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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Application Data (Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used to identify M2 macrophages in murine adipose tissue by immunofluorescence.Image caption:Expression of M2 markers CD206 and CD301 by ATMs of induced CLS and interstitial macrophages. M2 ATMs outside of CLS are highlighted by arrows. Scale bars?=?50?um.From: Lindhorst A, Raulien N, Wieghofer P, Eilers J, Rossi FMV, Bechmann I, Gericke M.Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.)

CD301, Monoclonal Antibody (Cat# AAA12294)

Full Name

RAT ANTI MOUSE CD301:RPE

Reactivity

Mouse

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-JCHAIN antibody (AAA19779).Overlay histogram showing A431 cells stained with AAA19779 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-JCHAIN Antibody (AAA19779, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of JCHAIN using anti-JCHAIN antibody (AAA19779).JCHAIN was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-JCHAIN Antibody (AAA19779) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-JCHAIN Antibody (AAA19779) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-JCHAIN Antibody (AAA19779) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse spleen tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JCHAIN antigen affinity purified polyclonal antibody (#AAA19779) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for JCHAIN at approximately 23 kDa. The expected band size for JCHAIN is at 18 kDa.)

JCHAIN, Polyclonal Antibody (Cat# AAA19779)

Full Name

Anti-JCHAIN Antibody Picoband

Gene Names

IGJ; JCH; IGCJ

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-SNRNP70 antibody (AAA19791).Overlay histogram showing HepG2 cells stained with AAA19791 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP70 Antibody (AAA19791, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of SNRNP70 using anti-SNRNP70 antibody (AAA19791) and anti-Beta Tubulin antibody (M01857-3).SNRNP70 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SNRNP70 Antibody (AAA19791) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP70 antigen affinity purified polyclonal antibody (#AAA19791) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SNRNP70 at approximately 95 kDa. The expected band size for SNRNP70 is at 52 kDa.)

SNRNP70, Polyclonal Antibody (Cat# AAA19791)

Full Name

Anti-SNRNP70 Antibody Picoband

Gene Names

SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-ASC/TMS1/PYCARD antibody (AAA19733).Overlay histogram showing THP-1 cells stained with AAA19733 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (AAA19733, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (AAA19733).ASC/TMS1/PYCARD was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ASC/TMS1/PYCARD Antibody (AAA19733) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ASC/TMS1/PYCARD Antibody (AAA19733) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HL-60 whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (#AAA19733) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.)

ASC/TMS1/PYCARD, Polyclonal Antibody (Cat# AAA19733)

Full Name

Anti-ASC/TMS1/PYCARD Antibody Picoband

Gene Names

PYCARD; ASC; TMS; TMS1; CARD5; TMS-1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)

TRIB2, Monoclonal Antibody (Cat# AAA24392)

Full Name

TRIB2 (Tribbles Homolog 2, TRB2, TRB-2, C5FW, GS3955) (AP)

Gene Names

TRIB2; C5FW; TRB2; GS3955

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in PC-12.)

Cyclophilin E, Monoclonal Antibody (Cat# AAA25659)

Full Name

Cyclophilin E (Cyclophilin 33, CYP33, CYP-33, MGC3736, MGC111222, Peptidyl-Prolyl Cis-Trans Isomerase E, PPIase E, PPIE, Rotamase E, Rotamase-E) (PE)

Gene Names

PPIE; CYP33; CYP-33

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FGFR1, Polyclonal Antibody (Cat# AAA28687)

Full Name

FGFR1 Antibody (Center)

Gene Names

FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1

Reactivity

Human, mouse (Predicted Reactivity: Chicken, Rat)

Applications

Flow Cytometry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using CASK antibody. Blue: DAPI for nuclear staining.)

CASK, Polyclonal Antibody (Cat# AAA29647)

Full Name

CASK Antibody

Gene Names

CASK; CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-ICAM-1 (Tyr512) Antibody. Lane 1: VERO cells, treated with blocking peptideLane 2: VERO cellsLane 3: A2780 cells.)

ICAM-1, Polyclonal Antibody (Cat# AAA30979)

Full Name

Phospho-ICAM-1 (Tyr512) Antibody

Gene Names

ICAM1; BB2; CD54; P3.58

Reactivity

Human, Mouse, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Pam212 cells, untreated or sulindac sulfone- treated (to induce apoptosis), using AAA14725 Results were similar to those obtained by analyzing DNA content.)

Caspase 3, Cleaved, Polyclonal Antibody (Cat# AAA14725)

Full Name

Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A and immunoaffinity chromatography.

Pricing

TNFalpha, ELISA Kit (Cat# AAA13515)

Full Name

TNFalpha Mouse ELISA Kit

Reactivity

Mouse

Applications

ELISA

Pricing

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IHC (Immunohistchemistry) (Detection of human SMARCA2/BRM by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-SMARCA/BRM recombinant monoclonal antibody (AAA23882). Secondary: HRP-conjugated goat anti-rabbit IgG .)

SMARCA2/BRM, Monoclonal Recombinant Antibody (Cat# AAA23882)

Full Name

Rabbit anti-SMARCA2/BRM Recombinant Monoclonal Antibody [BLR302M]

Gene Names

SMARCA2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

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Application Data

FGF1, Monoclonal Antibody (Cat# AAA25685)

Full Name

FGF1 (Fibroblast Growth Factor 1, FGF-1, Acidic Fibroblast Growth Factor, aFGF, Beta-endothelial Cell Growth Factor, ECGF-beta, Heparin-binding Growth Factor 1, HBGF-1, FGFA) (PE)

Gene Names

FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (F11R monoclonal antibody, Western Blot analysis of F11R expression in HepG2.)

Junctional Adhesion Molecule 1, Monoclonal Antibody (Cat# AAA25432)

Full Name

Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (HRP)

Gene Names

F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of Lyn using anti-Lyn antibody (AAA19150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lyn antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lyn at approximately 59KD. The expected band size for Lyn is at 59KD.)

Lyn, Polyclonal Antibody (Cat# AAA19150)

Full Name

Anti-Lyn Picoband Antibody

Gene Names

LYN; JTK8; p53Lyn; p56Lyn

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

Selenoprotein-P, Se-P, ELISA Kit (Cat# AAA11247)

Full Name

Human Selenoprotein-P, Se-P ELISA Kit

Gene Names

SELENOP; SeP; SELP; SEPP; SEPP1

Reactivity

Human

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RNF2 antibody. Western blot was performed from the immunoprecipitate using RNF2 antibody at a dilition of 1:1000.)

RNF2, Polyclonal Antibody (Cat# AAA10781)

Full Name

RNF2 Polyclonal Antibody

Gene Names

RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western Blot Analysis of human Jurkat cell lysate using CD31 Mouse Monoclonal Antibody (C31.7).)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13801)

Full Name

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human, Cynomolgus Monkey, Rabbit.
Weakly reacts with Rat

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of MEK2/MAP2K2 using anti-MEK2/MAP2K2 antibody (AAA19230).MEK2/MAP2K2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MEK2/MAP2K2 Antibody (AAA19230) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

MEK2/MAP2K2, Polyclonal Antibody (Cat# AAA19230)

Full Name

Anti-MEK2/MAP2K2 Antibody

Gene Names

MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

Histone H2A, Antibody (Cat# AAA17961)

Full Name

Histone H2A (Phospho-Thr121) Antibody

Gene Names

HIST1H2AI; H2A/c; H2AFC

Reactivity

Human (Identities = 100%, Positives = 100%)
Mouse (Identities = 100%, Positives = 100%)
Rat (Identities = 100%, Positives = 100%)

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

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Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:RPE)

CD8, Monoclonal Antibody (Cat# AAA12067)

Full Name

RAT ANTI DOG CD8:RPE

Applications

Flow Cytometry

Pricing

His-Streptavidin, Recombinant Protein (Cat# AAA11753)

Full Name

His-Streptavidin, 25-183aa, Streptomyces avidinii, His tag, E Coli

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

CCT7, Monoclonal Antibody (Cat# AAA25635)

Full Name

CCT7 (T-complex Protein 1 Subunit eta, TCP-1-eta, CCT-eta, HIV-1 Nef-interacting Protein, CCTH, NIP7-1) (PE)

Gene Names

CCT7; CCTH; CCTETA; NIP7-1; TCP1ETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with AKT1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

AKT1, Monoclonal Antibody (Cat# AAA30100)

Full Name

AKT1 Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using SUMO2/3 Rabbit mAb (AAA28605) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

SUMO2/3, Monoclonal Antibody (Cat# AAA28605)

Full Name

SUMO2/3 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

BCL-2, Monoclonal Antibody (Cat# AAA30284)

Full Name

BCL-2 Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Cytokeratin 18, Polyclonal Antibody (Cat# AAA31327)

Full Name

Phospho-Cytokeratin 18 (Ser51) Antibody

Gene Names

KRT18; K18; CYK18

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

PKC theta, Polyclonal Antibody (Cat# AAA31290)

Full Name

Phospho-PKC theta (Ser695) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-IGF-IR (Y1161) Polyclonal Antibody diluted at 1:500)

IGF-IR, Polyclonal Antibody (Cat# AAA30743)

Full Name

IGF-IR (Phospho-Tyr1161) Rabbit Polyclonal Antibody

Gene Names

IGF1R; IGFR; CD221; IGFIR; JTK13

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

C4a/b, Polyclonal Antibody (Cat# AAA29293)

Full Name

C4a/b Polyclonal Antibody

Gene Names

C4A; C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

MIF, Polyclonal Antibody (Cat# AAA29068)

Full Name

MIF Polyclonal Antibody

Gene Names

MIF; GIF; GLIF; MMIF

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using mH2A1 antibody.)

mH2A1, Polyclonal Antibody (Cat# AAA29836)

Full Name

mH2A1 Polyclonal Antibody

Gene Names

MACROH2A1; H2A.y; H2A/y; H2AFY; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MEK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

MEK3, Monoclonal Antibody (Cat# AAA30189)

Full Name

MEK3 Antibody

Gene Names

MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP, FC/FACS

Purity

ProA affinity purified

Pricing

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Low Endotoxin)

CD284, Monoclonal Antibody (Cat# AAA11902)

Full Name

MOUSE ANTI HUMAN CD284:Low Endotoxin

Gene Names

TLR4; TOLL; CD284; TLR-4; ARMD10

Applications

Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot

Pricing

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IL-1RL2, Polyclonal Antibody (Cat# AAA10970)

Full Name

IL-1RL2 Antibody

Gene Names

IL1RL2; IL-36R; IL1RRP2; IL-1Rrp2; IL1R-rp2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column.

Pricing

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CD83, Monoclonal Antibody (Cat# AAA12283)

Full Name

MOUSE ANTI HUMAN CD83

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Immunofluorescence

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEPG2 cells using anti-Alkaline phosphatase/ALPL antibody (AAA19231).Overlay histogram showing HEPG2 cells stained with AAA19231 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alkaline phosphatase/ALPL Antibody (AAA19231, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Alkaline phosphatase/ALPL, Polyclonal Antibody (Cat# AAA19231)

Full Name

Anti-Alkaline phosphatase/ALPL Antibody

Gene Names

ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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Integrin Beta 3 (ITGb3), Recombinant Protein (Cat# AAA20259)

Full Name

Recombinant Integrin Beta 3 (ITGb3)

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

>98%

Pricing

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WB (Western Blot) (Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody. Lane 1: ALB transfected lysate (69.4kD). Lane 2: Non-transfected lysate.)

ALB, Monoclonal Antibody (Cat# AAA25611)

Full Name

ALB (Albumin, Serum Albumin, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341) (PE)

Gene Names

ALB; HSA; PRO0883; PRO0903; PRO1341

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of GLUL expression in transfected 293T cell line by GLUL monoclonal antibody (M02), clone 3B6.Lane 1: GLUL transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.)

GLUL, Monoclonal Antibody (Cat# AAA26160)

Full Name

GLUL (Glutamate-Ammonia Ligase (Glutamine Synthetase), GLNS, GS, PIG43, PIG59) (Biotin)

Gene Names

GLUL; GS; GLNS; PIG43; PIG59

Applications

Western Blot

Purity

Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Smad2, Polyclonal Antibody (Cat# AAA29117)

Full Name

Smad2 Polyclonal Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)