Alpha Synuclein Oligomers (Dopamine HCL Stabilized) Recombinant Protein | SNCA recombinant protein

Human Recombinant Alpha Synuclein Oligomers (Dopamine HCL Stabilized)

Cat. Num. AAA27710

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Applications: Western Blot, SDS-Page
• Purity: >95%; Ion-exchange Purified. Monomeric alpha synuclein was removed via filtration.
• Synonyms: Alpha Synuclein Oligomers (Dopamine HCL Stabilized); N/A; Human Recombinant Alpha Synuclein Oligomers (Dopamine HCL Stabilized); Alpha Synuclein Oligomers; Alpha synuclein protein; Alpha-synuclein oligomer; Alpha synuclein protein oligomer; Alpha-synuclein protein; Non-A beta component of AD amyloid protein; Non-A4 component of amyloid precursor protein; NACP protein; SNCA protein; PARK1 protein; Alpha synuclein oligomers; Alpha Synuclein Protein Oligomers; SYN protein; Parkinson's disease familial 1 Protein; SNCA recombinant protein

• Host: E coli
• Purity/Purification: >95%; Ion-exchange Purified. Monomeric alpha synuclein was removed via filtration.
• Form/Format: PBS pH 7.4
• Concentration: Lot/batch specific. See included datasheet. (varies by lot)
• Sequence Length: Full length

• Applicable Applications for SNCA recombinant protein: Western Blot (WB), SDS-PAGE (SDS), In vivo assay, In vitro assay
• Species: Human
• Conjugation: No Tag
• Research Area: Neuroscience, Neurodegeneration, Alzheimer's Disease, Tangles & Tau, Neuroscience, Neurodegeneration, Parkinson's Disease, Synuclein, Neuroscience, Neurodegeneration, Multiple System Atrophy
• Cellular Localization: Cytoplasm, Membrane, Nucleus
• Certificate of Analysis: Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.
• Preparation and Storage: Store at -80 degree C

Application Data

(Coomassiegelstainofhumandopamine-stabilizedalphasynucleinoligomers(AAA27710).Load:2ug.)

Application Data

(Evaluationofa-syntoxicityonprimarymousecorticalneurons.Mitochondrialdehydrogenaseactivity,whichreducesyellowMTTtodarkblueformazancrystals,isareactionthatiscatalyzedinlivingcells.CellviabilitywasassessedwithanMTTassayanddisplayedas%ofvehiclecontrol(VC).Dataarepresentedasbargraphsandstandarddeviation.ForstatisticalanalysisOne-wayANOVAfollowedbyBonferronipost-hoctest(vsVC)wasused.**p)

Application Data

(Evaluationofa-syntoxicityonprimarymousecorticalneurons.Lactatedehydrogenase(LDH)isasolubleenzymepresentinthecytosolthatisreleaseduponcelldeath.ToxicitywasassessedwithanLDHassayanddisplayedas%ofvehiclecontrol(VC).Dataarepresentedasbargraphsandstandarddeviation.ForstatisticalanalysisOne-wayANOVAfollowedbyBonferronipost-hoctest(vsVC)wasused.***p)

Application Data

(HPLCofAlphaSynucleinProteinOligomers(AAA27710)randownasizeexclusioncolumn.Wavelength:280nm.)

Application Data

(TEMofHumanRecombinantAlphaSynucleinOligomers(DopamineHCLStabilized)(AAA27710))

Application Data

(TEMofHumanRecombinantAlphaSynucleinOligomers(DopamineHCLStabilized)(AAA27710))

Related Product Information for SNCA recombinant protein

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Dopamine inhibits alpha synuclein fibrillogenesis leading to oligomer accumulation in vitro (7), (8) and in vivo (9), (10).

Product Categories/Family for SNCA recombinant protein

Proteins

References

1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013).2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230.5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.7. Conway, K.A. et al. (2001) Science 294:1346-1349.8. Norris, E.H. et al. (2005) J Biol Chem. 280:21212-21219.9. Mazulli, J.R. et al. (2006). J Neurosci. 26:10068-10078.10. Mazulli, J.R. et al. (2007). J Biol Chem. 282:31621-31630.

NCBI and Uniprot Product Information

• NCBI GI #: 4507109
• NCBI GeneID: 6622
• NCBI Accession #: NP_000336.1
• NCBI GenBank Nucleotide #: NM_000345.3
• UniProt Accession #: P37840 ; Q13701; Q4JHI3; Q6IAU6; A8K2A4; P37840
• Molecular Weight: 14,460 Da
• NCBI Official Full Name: alpha-synuclein isoform NACP140
• NCBI Official Synonym Full Names: synuclein, alpha (non A4 component of amyloid precursor)
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein; synuclein alpha-140; non A-beta component of AD amyloid