Chicken alpha Synuclein Polyclonal Antibody | anti-SNCA antibody

alpha Synuclein

Cat. Num. AAA76715

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: alpha Synuclein, Antibody; alpha Synuclein; anti-SNCA antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
• Clonality: Polyclonal
• Isotype: Chicken IgY
• Form/Format: Liquid. Affinity purified antibody at 1mg/mL in 50% PBS, 50%glycerol plus 5mM NaN3

• Applicable Applications for anti-SNCA antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Full length recombinant human protein expressed in and purified from E coli
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, AAA76715, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.)

IF (Immunofluorescence)

(Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, AAA76715, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies.)

Related Product Information for anti-SNCA antibody

alpha-synuclein is a member of the synuclein protein family, the other two members being beta and gamma synuclein, each protein being coded for by a distinct but related gene. alpha-synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo, and direct homologues of alpha-synuclein are found in all vertebrates. Later work connected alpha-synuclein expression with several human brain pathologies, so that it is a major component of the Lewy bodies of Parkinson's disease. Point mutations of alpha-synuclein proved to be causative of some forms of familial Parkinson's disease. alpha-synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy and amyotrophic lateral sclerosis. alpha-synuclein is normally heavily expressed in brain and appears to be localized primarily to presynaptic regions, though not with a typical synaptic vesicle distribution pattern.

This antibody recognizes full length human and rodent alpha-synuclein specifically both in western blots and in immunocytochemical experiments and is a good marker of synapses on transgenic mice.

NCBI and Uniprot Product Information

• NCBI GI #: 4507109
• NCBI GeneID: 6622
• NCBI Accession #: NP_000336.1
• NCBI GenBank Nucleotide #: NM_000345.3
• UniProt Accession #: P37840; Q13701; Q4JHI3; Q6IAU6; A8K2A4
• Molecular Weight: 14,460 Da
• NCBI Official Full Name: alpha-synuclein isoform NACP140
• NCBI Official Synonym Full Names: synuclein, alpha (non A4 component of amyloid precursor)
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein; synuclein alpha-140; non A-beta component of AD amyloid
• UniProt Protein Name: Alpha-synuclein
• UniProt Gene Name: SNCA
• UniProt Synonym Gene Names: NACP; PARK1; NACP
• UniProt Entry Name: SYUA_HUMAN