Standard Curve (Sample)
Human amylase, alpha 2A (pancreatic) ELISA Kit | AMY2A elisa kit
Human Pancreatic Amylase, PAMY ELISA Kit
Gene Names
AMY2A; PA; AMY2; AMY2B
Reactivity
Human
Synonyms
amylase, alpha 2A (pancreatic); N/A; Human Pancreatic Amylase, PAMY ELISA Kit; Human Pancreatic Amylase; PAMY ELISA Kit; AMY2; AMY2B; PA; 1; 4-alpha-D-glucan glucanohydrolase; alpha-amylase; amylase; pancreatic; alpha-2A; found in the pancreas; glycogenase; pancreatic amylase 2A; pancreatic amylase alpha 2A; alpha 2A (pancreatic); AMY2A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human AMY2A. No significant cross-reactivity or interference between Human AMY2A and analogues was observed.
Sequence Length
511
Samples
Serum, plasma, tissue homogenates
Detection Range
46.88 mU/ml-3000 mU/ml
Sensitivity
11.7 mU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
glyconeogenesis and glycometabolism
Protein Biological Process 3
Carbohydrate metabolism
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AMY2A elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAMY. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PAMY and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PAMY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PAMY in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,707 Da
NCBI Official Full Name
pancreatic alpha-amylase
NCBI Official Synonym Full Names
amylase, alpha 2A (pancreatic)
NCBI Official Symbol
AMY2A
NCBI Official Synonym Symbols
PA; AMY2; AMY2B
NCBI Protein Information
pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase
UniProt Protein Name
Pancreatic alpha-amylase
UniProt Gene Name
AMY2A
UniProt Synonym Gene Names
PA
UniProt Entry Name
AMYP_HUMAN
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Product Notes
The Human AMY2A amy2a (Catalog #AAA79776) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79776 ELISA Kit recognizes Human AMY2A. It is sometimes possible for the material contained within the vial of "amylase, alpha 2A (pancreatic), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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