Standard Curve (Sample)
Mouse pancreatic alpha amylase (AMY2A) ELISA Kit | AMY2A elisa kit
Mouse pancreatic alpha amylase, AMY2A ELISA Kit
Gene Names
AMY2A; PA; AMY2; AMY2B
Reactivity
Mouse
Synonyms
pancreatic alpha amylase (AMY2A); N/A; Mouse pancreatic alpha amylase, AMY2A ELISA Kit; Mouse pancreatic alpha amylase (AMY2A) ELISA kit; AMY2; AMY2B; PA; 1; 4-alpha-D-glucan glucanohydrolase; alpha-amylase; amylase; pancreatic; alpha-2A; found in the pancreas; glycogenase; pancreatic amylase 2A; pancreatic amylase alpha 2A; AMY2A elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse AMY2A. No significant cross-reactivity or interference between mouse AMY2A and analogues was observed.
Sequence Length
511
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
9.38 mU/ml -600 mU/ml.
Sensitivity
The minimum detectable dose of mouse AMY2A is typically less than 2.3 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AMY2A elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMY2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMY2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMY2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMY2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,707 Da
NCBI Official Full Name
pancreatic alpha-amylase
NCBI Official Synonym Full Names
amylase, alpha 2A (pancreatic)
NCBI Official Symbol
AMY2A
NCBI Official Synonym Symbols
PA; AMY2; AMY2B
NCBI Protein Information
pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase
UniProt Protein Name
Pancreatic alpha-amylase
UniProt Gene Name
AMY2A
UniProt Synonym Gene Names
PA
UniProt Entry Name
AMYP_HUMAN
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Product Notes
The Mouse AMY2A amy2a (Catalog #AAA80949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80949 ELISA Kit recognizes Mouse AMY2A. It is sometimes possible for the material contained within the vial of "pancreatic alpha amylase (AMY2A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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