Standard Curve (Sample)
(Human Angiopoietin-1 PicoKine ELISA Kit standard curve)
Human Angiopoietin-1 ELISA Kit | ANGPT1 elisa kit
Human Angiopoietin-1 PicoKine ELISA Kit
Gene Names
ANGPT1; AGP1; AGPT; ANG1
Reactivity
Human
Synonyms
Angiopoietin-1; N/A; Human Angiopoietin-1 PicoKine ELISA Kit; angiopoietin 1; 1110046O21Rik; AGP 1; AGP1; AGPT; ANG 1; ANG1; Angiopoietin1; ANGPT 1; ANGPT1; KIAA0003; ANGPT1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Angiopoietin-1
Sequence Length
498
Samples
Cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human Angiopoietin-1 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Angiopoietin-1 PicoKine ELISA Kit standard curve)
Related Product Information for ANGPT1 elisa kit
Principle of the assay: human Angiopoietin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-1 has been precoated onto 96-well plates. Standards(NSO, H16-F498) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiopoietin-1 amount of sample captured in plate.
Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,456 Da
NCBI Official Full Name
Angiopoietin-1
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
ANGPT1
NCBI Official Synonym Symbols
AGP1; AGPT; ANG1
NCBI Protein Information
angiopoietin-1; ANG-1
UniProt Protein Name
Angiopoietin-1
UniProt Gene Name
ANGPT1
UniProt Synonym Gene Names
KIAA0003; ANG-1
UniProt Entry Name
ANGP1_HUMAN
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Product Notes
The Human ANGPT1 angpt1 (Catalog #AAA45926) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45926 ELISA Kit recognizes Human ANGPT1. It is sometimes possible for the material contained within the vial of "Angiopoietin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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