Rat Angiopoietin-1 ELISA Kit | ANGPT1 elisa kit

Rat Angiopoietin-1 PicoKine Quick ELISA Kit

Cat. Num. AAA128085

• Gene Names: Angpt1; Agpt; Agpt1; Ang-1
• Reactivity: Rat
• Applications: ELISA
• Synonyms: Angiopoietin-1; N/A; Rat Angiopoietin-1 PicoKine Quick ELISA Kit; ANGPT1 elisa kit

• Reactivity: Rat
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: H16-F498
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for ANGPT1 elisa kit: ELISA
• Kit Components: Description/Quantity/Volume; Anti-tag Pre-coated 96-well Strip Microplate/1/12 strips of 8 wells; Standard/2, 10 ng/tube; Antibody A/1/3ml; Antibody B/1/3ml; Sample Diluent/1/15ml; TBS-T Wash Buffer (25x)/1/12ml; Color Developing Reagent (TMB)/1/10ml; Stop Solution/1/10ml; Adhesive plate sealers/2/Piece
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Automated plate washer (optional); Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.; Horizontal orbital microplate shaker capable of maintaining a speed of 500rpm, amplitude 3mm.
• Sample Data:

  Concentration (pg/ml)	0	156	313	625	1250	2500	5000	10000
  O.D.	0.084	0.147	0.222	0.338	0.568	1.017	1.673	2.044

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	311	1285	4930	287	1289	5041
  Standard deviation	18.97	60.39	295.8	18.65	70.89	317.58
  CV (%)	6.1%	4.7%	6%	6.5%	5.5%	6.3%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	311	293	312	282	299	12.61	4.2%
  Sample 2	1285	1071	1253	1292	1225	90.26	7.3%
  Sample 3	4930	5240	5287	4371	4957	365.07	7.3%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Rat Angiopoietin-1 PicoKine Quick ELISA Kit standard curve)

Related Product Information for ANGPT1 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for ANGPT1 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 25014051
• NCBI GeneID: 89807
• UniProt Accession #: O35460; Q8K4Q4
• Molecular Weight: 57,461 Da
• NCBI Official Full Name: Angiopoietin-1
• NCBI Official Synonym Full Names: angiopoietin 1
• NCBI Official Symbol: Angpt1
• NCBI Official Synonym Symbols: Agpt; Agpt1; Ang-1
• NCBI Protein Information: angiopoietin-1
• UniProt Protein Name: Angiopoietin-1
• UniProt Gene Name: Angpt1
• UniProt Synonym Gene Names: ANG-1
• UniProt Entry Name: ANGP1_RAT