Standard Curve (Sample)
Rat Angiopoietin-1 ELISA Kit | ANGPT1 elisa kit
Rat Angiopoietin-1 ELISA Kit
Gene Names
Angpt1; Agpt; Agpt1; Ang-1
Reactivity
Rat
Applications
ELISA
Synonyms
Angiopoietin-1; N/A; Rat Angiopoietin-1 ELISA Kit; KIAA0003; ANG-1; ANGPT1 elisa kit
Reactivity
Rat
Specificity
The Rat Angiopoietin-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Angiopoietin-1 proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
497
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Applicable Applications for ANGPT1 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ANGPT1 elisa kit
Principle of the Assay||The Rat Angiopoietin-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Angiopoietin-1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat Angiopoietin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Angiopoietin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
Background/Introduction: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,461 Da
NCBI Official Full Name
Angiopoietin-1
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
Angpt1
NCBI Official Synonym Symbols
Agpt; Agpt1; Ang-1
NCBI Protein Information
angiopoietin-1
UniProt Protein Name
Angiopoietin-1
UniProt Gene Name
Angpt1
UniProt Synonym Gene Names
ANG-1
UniProt Entry Name
ANGP1_RAT
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Product Notes
The Rat ANGPT1 angpt1 (Catalog #AAA261665) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261665 ELISA Kit recognizes Rat ANGPT1. AAA Biotech's Angiopoietin-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the ANGPT1 angpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiopoietin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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