Standard Curve (Sample)
Mouse Atrial Natriuretic Peptide ELISA Kit | ANP elisa kit
Mouse Atrial Natriuretic Peptide ELISA Kit
Gene Names
NPPA; ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF
Reactivity
Mouse
Synonyms
Atrial Natriuretic Peptide; N/A; Mouse Atrial Natriuretic Peptide ELISA Kit; ANP/NPPA/ANF/Cardionatrin/NPPA/PND/ANF/ANPatriopeptin/ATFB6/atrial natriuretic factor/Atrial natriuretic peptide/CDD-ANF/natriuretic peptide A/natriuretic peptide precursor A/PNDcardionatrin/prepronatriodilatin/Prepronatriodilatin; ANP elisa kit
Reactivity
Mouse
Specificity
Specifically recognize ANP, no obvious cross reaction with other analogues
Sequence Length
151
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ANP elisa kit
Background: Atrial natriuretic polypeptide, alpha (ANP), atrial natriuretic factor (ANF), or atriopeptin, is a protein (polypeptide) hormone secreted by heart muscle cells. It is involved in the homeostatic control of body water, sodium, potassium and fat (adiposity). It is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes), in response to high blood pressure. ANP acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. Atrial Natriuretic Peptide has the potential for cardiovascular diseases research.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ANP antibody was precoated onto the 96-well plate. The biotin conjugated anti ANP antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ANP conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ANP in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ANP antibody was precoated onto the 96-well plate. The biotin conjugated anti ANP antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ANP conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ANP in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,708 Da
NCBI Official Full Name
atrial natriuretic peptide
NCBI Official Synonym Full Names
natriuretic peptide A
NCBI Official Symbol
NPPA
NCBI Official Synonym Symbols
ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF
NCBI Protein Information
natriuretic peptides A
UniProt Protein Name
Natriuretic peptides A
UniProt Gene Name
NPPA
UniProt Synonym Gene Names
ANP; PND; CDD; CDP; ANF; ANP
UniProt Entry Name
ANF_HUMAN
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Product Notes
The Mouse ANP nppa (Catalog #AAA102289) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA102289 ELISA Kit recognizes Mouse ANP. It is sometimes possible for the material contained within the vial of "Atrial Natriuretic Peptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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