Human Annexin A1 ELISA Kit | ANXA1 elisa kit

Human Annexin A1 Quick ELISA Kit

Cat. Num. AAA125896

• Gene Names: ANXA1; ANX1; LPC1
• Reactivity: Human
• Synonyms: Annexin A1; N/A; Human Annexin A1 Quick ELISA Kit; Annexin A1|Annexin A1; annexin I (lipocortin I); Annexin I; Annexin-1; ANX1; ANXA1; Calpactin II; calpactin-2; chromobindin-9; Lipocortin I; LPC1; LPC1annexin-1; p35; Phospholipase A2 inhibitory protein; ANXA1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human ANXA1
• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <100pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.056|0.128|0.173 |0.274|0.458|0.833|1.436|2.197
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|288|1452|5263|; Standard deviation|12.67|59.53|268.41|; CV(%)|4.4%|4.1%|5.1%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|301|1563|5364; Standard deviation|17.16|100.03|413.03; CV(%)|5.7%|6.4%|7.7%

• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 288|291|294|288|290|6.39|2.2%; Sample 2: 1452|1426|1367|1348|1398|27.97|2%; Sample 3: 5263|4796|5102|4982|5036|125.89|2.5%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A2-N346
• Anticoagulant: heparin, EDTA or citrate
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human Annexin A1 with <100pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human ANXA1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human ANXA1 with immunogen: Expression system for standard: NS0, Immunogen sequence: A2-N346.; ; To measure Human ANXA1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ANXA1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ANXA1 in the sample.
• Assay Procedure Step 1: 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312.5pg/ml, 156.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Application Data

Related Product Information for ANXA1 elisa kit

ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21. 13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Product Categories/Family for ANXA1 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NM_000700.1
• UniProt Accession #: P04083
• Molecular Weight: 38,714 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1
• UniProt Entry Name: ANXA1_HUMAN