WB (Western Blot)
(Detection of human and mouse ANXA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ANXA1 antibody AAA213164 (lot AAA213164-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse ANXA1/Annexin A1 Polyclonal Antibody | anti-ANXA1 antibody
Rabbit anti-ANXA1/Annexin A1 Antibody, Affinity Purified
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ANXA1/Annexin A1, Antibody; Rabbit anti-ANXA1/Annexin A1 Antibody, Affinity Purified; calpactin-2; phospholipase A2 inhibitory protein; p35; LPC1; Lipocortin I; chromobindin-9; calpactin II; ANX1; annexin-1; annexin I (lipocortin I); Annexin I; annexin A1; epididymis secretory sperm binding protein; Annexin A1; anti-ANXA1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ANXA1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine, Horse, Orangutan, Chimpanzee
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ANXA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ANXA1 antibody AAA213164 (lot AAA213164-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human and mouse ANXA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ANXA1 antibody AAA213164 (lot AAA213164-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ANXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ANXA1 antibody AAA213164 (lot AAA213164-1) used for IP at 6 ug per reaction. ANXA1 was also immunoprecipitated by rabbit anti-ANXA1 antibody For blotting immunoprecipitated ANXA1, AAA213164 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ANXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ANXA1 antibody AAA213164 (lot AAA213164-1) used for IP at 6 ug per reaction. ANXA1 was also immunoprecipitated by rabbit anti-ANXA1 antibody For blotting immunoprecipitated ANXA1, AAA213164 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human ANXA1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-ANXA1 (Cat. No. AAA213164 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ANXA1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-ANXA1 (Cat. No. AAA213164 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-ANXA1 antibody
Annexin A1 is a membrane-localized protein that binds phospholipids. It inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression has been detected in multiple tumors [taken from NCBI Entrez Gene (Gene ID: 301)].
Product Categories/Family for anti-ANXA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,714 Da
NCBI Official Full Name
annexin A1
NCBI Official Synonym Full Names
annexin A1
NCBI Official Symbol
ANXA1
NCBI Official Synonym Symbols
ANX1; LPC1
NCBI Protein Information
annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein
UniProt Protein Name
Annexin A1
UniProt Gene Name
ANXA1
UniProt Synonym Gene Names
ANX1; LPC1
UniProt Entry Name
ANXA1_HUMAN
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Product Notes
The ANXA1 anxa1 (Catalog #AAA213164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ANXA1/Annexin A1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ANXA1/Annexin A1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ANXA1 anxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANXA1/Annexin A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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