SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Apoa1 recombinant protein
Apoa1, 25-264aa, Mouse, His tag, E Coli
Gene Names
Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Apoa1; N/A; Apoa1, 25-264aa, Mouse, His tag, E Coli; Apolipoprotein A-I; Alp-1; apo-AI; Apoa-1; apoA-I; Brp-14,Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2; Apolipoprotein AI; Apoa1 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid, In Phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM DTT
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSDEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ
Sequence Length
264
Applicable Applications for Apoa1 recombinant protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Domain
25-264aa
Preparation and Storage
Can be stored at +2°C to +8°C for one week. For long term storage , aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for Apoa1 recombinant protein
Apoa1 also known as Apolipoprotein A-I, is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apoa1 are associated with HDL deficiency including Tangier disease. Recombinant mouse Apoa1, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for Apoa1 recombinant protein
References
Sontag TJ., et al. (2014) Arterioscler Thromb Vasc Biol, 34 (2), 262-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30.3kDa (263aa) confirmed by MALDI-TOF
NCBI Official Full Name
apolipoprotein A-I preproprotein
NCBI Official Synonym Full Names
apolipoprotein A-I
NCBI Official Symbol
Apoa1
NCBI Official Synonym Symbols
Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
NCBI Protein Information
apolipoprotein A-I
UniProt Protein Name
Apolipoprotein A-I
UniProt Gene Name
Apoa1
UniProt Synonym Gene Names
Apo-AI; ApoA-I; ProapoA-I
Similar Products
Product Notes
The Apoa1 apoa1 (Catalog #AAA11832) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Apoa1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Apoa1 apoa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSDEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ. It is sometimes possible for the material contained within the vial of "Apoa1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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