SDS-PAGE
Apolipoprotein E (ApoE) Recombinant Protein | ApoE recombinant protein
Recombinant Human Apolipoprotein E (ApoE) Protein
Gene Names
APOE; AD2; LPG; APO-E; ApoE4; LDLCQ5
Applications
ELISA, Western Blot
Purity
> 90 % as determined by SDS-PAGE.
Synonyms
Apolipoprotein E (ApoE); N/A; Recombinant Human Apolipoprotein E (ApoE) Protein; Apo-E; APOE; ApoE; ApoE recombinant protein
Host
Mammalian Cells 1-317 AA (P02649).
Purity/Purification
> 90 % as determined by SDS-PAGE.
Form/Format
Supplied in 20 mM PB, 150 mM NaCl, pH 7.4.
Concentration
0.5 mg/mL (varies by lot)
Sequence
MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQ VTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQS TEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQ ERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQW AGLVEKVQAAVGTSAAPVPSDNH
Sequence Length
317
Applicable Applications for ApoE recombinant protein
ELISA, WB (Western Blot)
Source
Human
Protein Residues
C-terminal 6X His-tagged.
Usage
ApoE Protein - Centrifuge the standard vial at 6000-10000rpm for 30s.
Preparation and Storage
Store it under sterile conditions at -20°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. The recombinant protein is stable for up to 12 months from date of receipt at -80°C. **Avoid repeated freeze-thaw cycles.**
SDS-PAGE
SDS-PAGE
Related Product Information for ApoE recombinant protein
Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Apolipoprotein E has many functions in the body. When it is synthesized by the liver as part of VLDL it functions in the transport of triglycerides to the liver tissue. It is also incorporated into HDL (as HDL-E) and functions in cholesterol distribution among cells. It is also incorporated into intestinally synthesized cholymicrons and transports dietary triglycerides and cholesterol. It is involved in lipid metabolism by mediating the receptor binding of apo-E lipoproteins to the LDL receptor. Receptor binding begins the cellular uptake of lipoproteins to be used in intracellular cholesterol metabolism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 50 kDa
NCBI Official Full Name
apolipoprotein E isoform b
NCBI Official Synonym Full Names
apolipoprotein E
NCBI Official Symbol
APOE
NCBI Official Synonym Symbols
AD2; LPG; APO-E; ApoE4; LDLCQ5
NCBI Protein Information
apolipoprotein E
UniProt Protein Name
Apolipoprotein E
UniProt Gene Name
APOE
UniProt Synonym Gene Names
Apo-E
UniProt Entry Name
APOE_HUMAN
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Product Notes
The ApoE apoe (Catalog #AAA55951) is a Recombinant Protein produced from Mammalian Cells 1-317 AA (P02649). and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Apolipoprotein E (ApoE) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ApoE apoe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKVLWAALLV TFLAGCQAKV EQAVETEPEP ELRQQTEWQS GQRWELALGR FWDYLRWVQT LSEQVQEELL SSQ VTQELRALMD ETMKELKAYK SELEEQLTPV AEETRARLSK ELQAAQARLG ADMEDVCGRL VQYRGEVQAM LGQS TEELRVRLAS HLRKLRKRLL RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQ ERAQAWGERL RARMEEMGSR TRDRLDEVKE QVAEVRAKLE EQAQQIRLQA EAFQARLKSW FEPLVEDMQR QW AGLVEKVQAA VGTSAAPVPS DNH. It is sometimes possible for the material contained within the vial of "Apolipoprotein E (ApoE), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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