Application Data
(HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SETD1B-SET recombinant protein
Recombinant SETD1B-SET complex
Gene Names
ASH2L; ASH2; Bre2; ASH2L1; ASH2L2
Synonyms
SETD1B-SET; N/A; Recombinant SETD1B-SET complex; Histone-lysine N-methyltransferase SETD1B; SETD1B-SET recombinant protein
Host
Baculovirus
Form/Format
Recombinant SETD1B-SET Complex is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
Protein Species
Human
Notes
This complex is suitable for use in protein-protein interaction, in vitro transcription assay, binding assays.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for SETD1B-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1B-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant SETD1B-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1B-SET: 35.7 kDa MW of WDR5: 36.6 kDa MW of RBBP5: 59.2 kDa MW of ASH2L: 68.7 kDa Purity: >90%)
SDS-PAGE
(Recombinant SETD1B-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1B-SET: 35.7 kDa MW of WDR5: 36.6 kDa MW of RBBP5: 59.2 kDa MW of ASH2L: 68.7 kDa Purity: >90%)
Related Product Information for SETD1B-SET recombinant protein
Short Description: Recombinant SETD1B-SET Complex that includes amino acids 1669-1966 of human SETD1B protein (accession number NP_001340274.1) with a N-terminal DYKDDDDK tag and full-length human WDR5 protein (accession number NP_060058.1) without tag and full-length human RBBP5 protein (accession number NP_005048.2) without tag and full-length human ASH2L protein (accession number NP_001098684.1) without tag was expressed in Sf9 cells. The molecular weights of SETD1A-SET, WDR5, RBBP5,ASH2L are 35.7 kDa, 36.6 kDa, 59.2 kDa and 68.7 kDa, respectively. This complex is suitable for use in protein-protein interaction, in vitro transcription assay, binding assays.
Background: SETD1B (SET Domain Containing 1B, Histone Lysine Methyltransferase) is a component of a histone methyltransferase (HMT) complex (contain SETD1B-SET ASH2L, RBBP5, WDR5) that produces mono-, di-, and trimethylated histone H3 at Lys4, but not if the neighboring 'Lys-9' residue is already methylated. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily.
Background: SETD1B (SET Domain Containing 1B, Histone Lysine Methyltransferase) is a component of a histone methyltransferase (HMT) complex (contain SETD1B-SET ASH2L, RBBP5, WDR5) that produces mono-, di-, and trimethylated histone H3 at Lys4, but not if the neighboring 'Lys-9' residue is already methylated. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily.
Product Categories/Family for SETD1B-SET recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,208 Da
NCBI Official Full Name
set1/Ash2 histone methyltransferase complex subunit ASH2 isoform b
NCBI Official Synonym Full Names
ash2 (absent, small, or homeotic)-like (Drosophila)
NCBI Official Symbol
ASH2L
NCBI Official Synonym Symbols
ASH2; Bre2; ASH2L1; ASH2L2
NCBI Protein Information
set1/Ash2 histone methyltransferase complex subunit ASH2
UniProt Protein Name
Set1/Ash2 histone methyltransferase complex subunit ASH2
UniProt Gene Name
ASH2L
UniProt Synonym Gene Names
ASH2L1
UniProt Entry Name
ASH2L_HUMAN
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Product Notes
The SETD1B-SET ash2l (Catalog #AAA60223) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SETD1B-SET, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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