Human asporin ELISA Kit | ASPN elisa kit

Human Asporin, ASPN ELISA Kit

Cat. Num. AAA112818

• Gene Names: ASPN; OS3; PLAP1; PLAP-1; SLRR1C
• Reactivity: Human
• Synonyms: asporin; N/A; Human Asporin, ASPN ELISA Kit; Human Asporin (ASPN) ELISA kit; FLJ20129; OS3; PLAP1; SLRR1C; asporin (LRR class 1) ; asporin proteoglycan; periodontal ligament associated protein 1; small leucine-rich protein 1C; ASPN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ASPN. No significant cross-reactivity or interference between human ASPN and analogues was observed.
• Sequence Length: 242
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 34.37 pg/ml -2200 pg/ml.
• Sensitivity: The minimum detectable dose of human ASPN is typically less than 8.59 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for ASPN elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ASPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ASPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASPN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASPN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 301069324
• NCBI GeneID: 54829
• NCBI Accession #: NP_001180264.1
• NCBI GenBank Nucleotide #: NM_001193335.1
• UniProt Accession #: Q9BXN1; Q5TBF3; Q96K79; Q96LD0; Q9NXP3
• Molecular Weight: 43,417 Da
• NCBI Official Full Name: asporin isoform 2 preproprotein
• NCBI Official Synonym Full Names: asporin
• NCBI Official Symbol: ASPN
• NCBI Official Synonym Symbols: OS3; PLAP1; PLAP-1; SLRR1C
• NCBI Protein Information: asporin; asporin proteoglycan; asporin (LRR class 1); small leucine-rich protein 1C; periodontal ligament associated protein 1
• UniProt Protein Name: Asporin
• UniProt Gene Name: ASPN
• UniProt Synonym Gene Names: PLAP1; SLRR1C; UNQ215/PRO241; PLAP-1
• UniProt Entry Name: ASPN_HUMAN