Standard Curve (Sample)
Adenosine Monophosphate Assay Kit
Adenosine Monophosphate Assay Kit
Synonyms
Adenosine Monophosphate; N/A; Adenosine Monophosphate Assay Kit; Adenosine Monophosphate assay kit
Detection Method
Fluorometric
Preparation and Storage
Upon receipt, store PPDK at -80°C and the rest of the components at -20ºC. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Adenosine Monophosphate assay kit
Background: Adenosine monophosphate (AMP) is a nucleotide made up of a phosphate group, the sugar ribose, and the nucleobase adenine. AMP is an important part of various metabolic reactions since AMP is often converted to adenosine diphosphate (ADP) or adenosine triphosphate (ATP). AMP is a constituent of RNA strand synthesis. AMP regulates AMP-activated protein kinase (AMPK), an enzyme that controls cell migration, calcium signaling, and the secretion of cytokines, and t-cell priming capacity in immune cells such as dendritic cells. The Adenosine Monophosphate Assay Kit is a simple fluorometric assay that measures the amount of adenosine monophosphate present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, adenosine monophosphate standards, and unknown samples. Sample adenosine monophosphate concentrations are determined by comparison with a known adenosine monophosphate standard. The kit has a detection sensitivity limit of 1.6 uM adenosine monophosphate.
Principle of the Assay: The Adenosine Monophosphate Assay Kit measures total adenosine monophosphate within biological samples. Adenosine monophosphate (with pyrophosphate and phosphoenolpyruvate) is converted by PPDK to pyruvate + adenosine triphosphate (ATP) and phosphate. Pyruvate is converted by pyruvate oxidase in the presence of phosphate and oxygen into acetyl phosphate, carbon dioxide, and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of adenosine monophosphate standard within the 96-well microtiter plate format. Samples and standards are incubated for 30 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Principle of the Assay: The Adenosine Monophosphate Assay Kit measures total adenosine monophosphate within biological samples. Adenosine monophosphate (with pyrophosphate and phosphoenolpyruvate) is converted by PPDK to pyruvate + adenosine triphosphate (ATP) and phosphate. Pyruvate is converted by pyruvate oxidase in the presence of phosphate and oxygen into acetyl phosphate, carbon dioxide, and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of adenosine monophosphate standard within the 96-well microtiter plate format. Samples and standards are incubated for 30 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
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Product Notes
The Adenosine Monophosphate (Catalog #AAA44690) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Adenosine Monophosphate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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