Comet Assay Kit
Comet Assay Kit
Synonyms
Comet; N/A; Comet Assay Kit; DNA damage; Comet assay kit
Samples
Tissue, Cells
Usage Note
Do not mix or substitute reagents or materials from other kit lots or vendors.
Preparation and Storage
Stored at 4 degree C for 6 months, protected from light
Related Product Information for Comet assay kit
Principle of the Assay: Comet Assay Kit (3-Well Slides) is a single cell gel electrophoresis assay (SCGE), rapid and sensitive test for detecting DNA damage in cells. The principle is that after organs or tissues have been suffered from (such as radiation, heavy metals, etc.), single strand or double strand DNA was broken in the cells, the cell is mixed with melted agarose and added on a slide, then the cell membrane is disrupted with lysis solution, the DNA is uncoiled with alkaline solution. Finally, the samples were electrophoresed, under the action of an electric field, the macromolecular DNA of normal cells migrates for a shorter distance, intact DNA remains in the range of the nucleus, broken pieces of DNA migrate out of the nucleus, stained with PI dye, observed under a fluorescence microscope, intact DNA forms a circular or slightly tailed pattern; DNA fragmentation fragments form comet-like pattern. According to the pH value of the electrophoresis buffer, it can be divided into neutral comet assay (pH=8.4) and alkaline comet assay (pH>13). Neutral comet assay was mainly used to detect the broken damage of DNA double strand, and alkaline comet assay has higher sensitivity and can be used to detect less single strand and double strand broken damage.
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Product Notes
The Comet (Catalog #AAA332621) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Comet, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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