Standard Curve (Sample)
CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric Assay Kit
CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric
Synonyms
CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric; N/A; CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric assay kit
Preparation and Storage
Store all components at 4 degree C until their expiration dates.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric assay kit
Principle of the Assay: The CytoSelect™ Cell Migration Assay Kit contains polycarbonate membrane inserts (8 um pore size) in a 24-well plate. The membrane serves as a barrier to discriminate migratory cells from non-migratory cells. Migratory cells are able to extend protrusions towards chemoattractants (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. Finally, the cells are removed from the top of the membrane and the migratory cells are stained and quantified.
Background/Introduction: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 um pore size) to assay the migratory properties of cells. The kit contains sufficient reagents for the evaluation of 12 samples. The 8 um pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 um) is recommended.
Background/Introduction: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 um pore size) to assay the migratory properties of cells. The kit contains sufficient reagents for the evaluation of 12 samples. The 8 um pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 um) is recommended.
Product Categories/Family for CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric assay kit
References
1. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. (2003) Science 302, 1704-9.
2. Horwitz R, Webb D. (2003) Curr Biol. 13, R756-9.
3. Lauffenburger DA, Horwitz AF. (1996) Cell 84, 359-369.
1. Kanan, Y. et al. (2014). Fibulin 2, a tyrosine O-sulfated protein, is up-regulated following retinal detachment. J Biol Chem. 289:13419-13433.
2. Al-Wadei, M.H. et al. (2013).Gamma-amino Butyric Acid (GABA) prevents the induction of nicotinic receptor-regulated signaling by chronic ethanol in pancreatic cancer cells and normal duct epithelia. Cancer Prevention Research. 6:139-148.
3. Al-Wadei, M.H. et al. (2012).Effects of chronic nicotine on the autocrine regulation of pancreatic cancer cells and pancreatic duct epithelial cells by stimulatory and inhibitory neurotransmitters. Carcinogenesis. 33:1745-7153.
4. Chen, Z. et al. (2012). The iron chelators Dp44mT and DFO inhibit TGF-beta-induced epithelial-mesenchymal transition via up-regulation of N-Myc downstream-regulated gene 1 (NDRG1). J.Biol.Chem. 287:17016-17028.
5. Isakova, I. et al. (2007). Age and dose-related effects on MSC engraftment levels and anatomical distribution in the CNS of non-human primates: Identification of novel MSC subpopulations that respond to guidance cues in brain. Stem Cells 25: 3261-3270.
6. Schuller, H. et al. (2007). GABA B receptor is a novel drug target for pancreatic cancer. Cancer 112(4):767-768.
7. Eckstein, N. et al. (2008). EGFR-pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem. 283:739-750.
8. Liu, S. et al. (2008). Matrix metalloproteinase-activated anthrax lethal toxin demonstrates high potency in targeting tumor vasculature. J. Biol. Chem. 283:529-540.
9. Awasthi, N. et al. (2008). Downregulation of MMP-2 and -9 by proteasome inhibition: A possible mechanism to decrease LEC migration and prevent posterior capsular opacification. Invest. Ophthalmol. Vis. Sci. 49:1998-2003.
10. Ma, J. et al. (2008). Pancreatic duodenal homeobox-1(PDX-1) functions as a tumor suppressor in gastric cancer. Carcinogenesis 29:1327-1333.
11. Lukas, T.J. et al. (2008). Susceptibility to glaucoma: differential comparison of the astrocyte transcriptome from glaucomatous African American and Caucasian American donors. Genome Biology 9:R111.
12. Schuller, H. et al. (2008). Gamma-aminobutyric acid, a potential tumor suppressor for small airway-derived lung adenocarcinoma. Carcinogenesis 29:1979-1985.
13. Awasthi, N. et al. (2009). Endothelial monocyte activating polypeptide II interferes with VEGF-induced proangiogenic signaling. Laboratory Investigation 89(1):38-46.
14. Tabata, C. et al. (2009). All-trans-retinoic acid inhibits tumor growth of malignant pleural mesothelioma in mice. Eur. Respir. J. 34:1159-1167.
15. Igarashi, J. et al. (2009). Transforming growth factor-beta1 downregulates caveolin-1 expression and enhances sphingosine 1-phosphate signaling in cultured vascular endothelial cells. Am. J. Physiol. Cell Physiol. 297:C1263-C1274.
16. Tabata, C. et al. (2010). Novel clinical role of angiopoietin-1 in malignant pleural mesothelioma. Eur. Respir. J. 10.1183/09031936.00154009.
2. Horwitz R, Webb D. (2003) Curr Biol. 13, R756-9.
3. Lauffenburger DA, Horwitz AF. (1996) Cell 84, 359-369.
1. Kanan, Y. et al. (2014). Fibulin 2, a tyrosine O-sulfated protein, is up-regulated following retinal detachment. J Biol Chem. 289:13419-13433.
2. Al-Wadei, M.H. et al. (2013).Gamma-amino Butyric Acid (GABA) prevents the induction of nicotinic receptor-regulated signaling by chronic ethanol in pancreatic cancer cells and normal duct epithelia. Cancer Prevention Research. 6:139-148.
3. Al-Wadei, M.H. et al. (2012).Effects of chronic nicotine on the autocrine regulation of pancreatic cancer cells and pancreatic duct epithelial cells by stimulatory and inhibitory neurotransmitters. Carcinogenesis. 33:1745-7153.
4. Chen, Z. et al. (2012). The iron chelators Dp44mT and DFO inhibit TGF-beta-induced epithelial-mesenchymal transition via up-regulation of N-Myc downstream-regulated gene 1 (NDRG1). J.Biol.Chem. 287:17016-17028.
5. Isakova, I. et al. (2007). Age and dose-related effects on MSC engraftment levels and anatomical distribution in the CNS of non-human primates: Identification of novel MSC subpopulations that respond to guidance cues in brain. Stem Cells 25: 3261-3270.
6. Schuller, H. et al. (2007). GABA B receptor is a novel drug target for pancreatic cancer. Cancer 112(4):767-768.
7. Eckstein, N. et al. (2008). EGFR-pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem. 283:739-750.
8. Liu, S. et al. (2008). Matrix metalloproteinase-activated anthrax lethal toxin demonstrates high potency in targeting tumor vasculature. J. Biol. Chem. 283:529-540.
9. Awasthi, N. et al. (2008). Downregulation of MMP-2 and -9 by proteasome inhibition: A possible mechanism to decrease LEC migration and prevent posterior capsular opacification. Invest. Ophthalmol. Vis. Sci. 49:1998-2003.
10. Ma, J. et al. (2008). Pancreatic duodenal homeobox-1(PDX-1) functions as a tumor suppressor in gastric cancer. Carcinogenesis 29:1327-1333.
11. Lukas, T.J. et al. (2008). Susceptibility to glaucoma: differential comparison of the astrocyte transcriptome from glaucomatous African American and Caucasian American donors. Genome Biology 9:R111.
12. Schuller, H. et al. (2008). Gamma-aminobutyric acid, a potential tumor suppressor for small airway-derived lung adenocarcinoma. Carcinogenesis 29:1979-1985.
13. Awasthi, N. et al. (2009). Endothelial monocyte activating polypeptide II interferes with VEGF-induced proangiogenic signaling. Laboratory Investigation 89(1):38-46.
14. Tabata, C. et al. (2009). All-trans-retinoic acid inhibits tumor growth of malignant pleural mesothelioma in mice. Eur. Respir. J. 34:1159-1167.
15. Igarashi, J. et al. (2009). Transforming growth factor-beta1 downregulates caveolin-1 expression and enhances sphingosine 1-phosphate signaling in cultured vascular endothelial cells. Am. J. Physiol. Cell Physiol. 297:C1263-C1274.
16. Tabata, C. et al. (2010). Novel clinical role of angiopoietin-1 in malignant pleural mesothelioma. Eur. Respir. J. 10.1183/09031936.00154009.
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Product Notes
The CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric (Catalog #AAA11404) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect 24-well Cell Migration Assay (8 um), Colorimetric, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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