Application Data
(Test: Jurkat cells were treated with 10 uM EdU for 4 hours. Negative: Jurkat cells without EdU.)
EdU Cell Proliferation Flow Cytometry Assay Kit
E-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Green, AF488)
Synonyms
EdU Cell Proliferation Flow Cytometry; N/A; E-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Green, AF488); EdU Cell Proliferation Flow Cytometry assay kit
Form/Format
PBS buffer (with 1%BSA) (PH7.2 ~ 7.4), PBS (with 1% Saponin) (pH7.2~7.6), 4% Polyformaldehyde (dissolved in PBS)(E-IR-R113), Deionized water.
Samples
Cell samples
Detection Instrument
Flow Cytometry
Dye Type
Fluor 488
Channel Set
FITC
Detection Principle
Cell proliferation assays are widely used in the evaluation of cell viability, genotoxicity, and the effect of antitumor drugs. Direct detection of DNA synthesis in cells is considered to be the most accurate method for detecting cell proliferation. The initial widely used method for detecting DNA synthesis in cells was the radiolabeled nucleoside incorporation method, but this method was greatly limited due to radioactive contamination and the difficulty of single-cell detection, and was gradually replaced by the BrdU method based on antibody detection. The BrdU method has many steps and requires the use of BrdU antibody, which has many influencing factors and poor stability. EdU method is based on EdU incorporation and subsequent click reaction, without the use of antibodies, convenient operation and high detection sensitivity. It is a new method upgraded on the basis of BrdU method and will gradually replace BrdU method. EdU (5-ethynyl-2-deoxyuridine), is a thymidine analog, EdU can replace thymidine in the process of DNA synthesis to incorporate into new in synthetic DNA. On the other hand, the acetylene group on EdU can react with fluorescently labeled small molecule azide probes (such as FITC Azide, Fluor 488 Azide, Fluor 594 Azide, Fluor 647 Azide) through the catalysis of monovalent copper ions to form a stable triazole ring. This reaction is very rapid and is called the click reaction. Through the click reaction, the newly synthesized DNA is labeled with the corresponding fluorescent probe, so that the proliferating cells can be detected using the appropriate fluorescent detection equipment.
Preparation and Storage
Store at -20 degree C, shading light
Estimated Expiration: 12 months
Shipping: Ice bag
Estimated Expiration: 12 months
Shipping: Ice bag
Application Data
(Test: Jurkat cells were treated with 10 uM EdU for 4 hours. Negative: Jurkat cells without EdU.)
Application Data
(Test: Jurkat cells were treated with 10 uM EdU for 4 hours. Negative: Jurkat cells without EdU.)
Application Data
(Test: HL-60 cells were treated with 10 uM EdU for 3 hours. Negative: HL-60 cells without EdU.)
Application Data
(Test: HL-60 cells were treated with 10 uM EdU for 3 hours. Negative: HL-60 cells without EdU.)
Related Product Information for EdU Cell Proliferation Flow Cytometry assay kit
Background: E-Click EdU Cell Proliferation Flow Cytometry Assay Kit is easy to operate and has high sensitivity. It is suitable for the proliferation assay of suspension cells, and the results can be analyzed by flow cytometry.
Principle of the Assay: Cell proliferation assays are widely used in the evaluation of cell viability, genotoxicity, and the effect of antitumor drugs. Direct detection of DNA synthesis in cells is considered to be the most accurate method for detecting cell proliferation. The initial widely used method for detecting DNA synthesis in cells was the radiolabeled nucleoside incorporation method, but this method was greatly limited due to radioactive contamination and the difficulty of single-cell detection, and was gradually replaced by the BrdU method based on antibody detection. The BrdU method has many steps and requires the use of BrdU antibody, which has many influencing factors and poor stability. EdU method is based on EdU incorporation and subsequent click reaction, without the use of antibodies, convenient operation and high detection sensitivity. It is a new method upgraded on the basis of BrdU method and will gradually replace BrdU method. EdU (5-ethynyl-2-deoxyuridine), is a thymidine analog, EdU can replace thymidine in the process of DNA synthesis to incorporate into new in synthetic DNA. On the other hand, the acetylene group on EdU can react with fluorescently labeled small molecule azide probes (such as FITC Azide, Fluor 488 Azide, Fluor 594 Azide, Fluor 647 Azide) through the catalysis of monovalent copper ions to form a stable triazole ring. This reaction is very rapid and is called the click reaction. Through the click reaction, the newly synthesized DNA is labeled with the corresponding fluorescent probe, so that the proliferating cells can be detected using the appropriate fluorescent detection equipment.
Principle of the Assay: Cell proliferation assays are widely used in the evaluation of cell viability, genotoxicity, and the effect of antitumor drugs. Direct detection of DNA synthesis in cells is considered to be the most accurate method for detecting cell proliferation. The initial widely used method for detecting DNA synthesis in cells was the radiolabeled nucleoside incorporation method, but this method was greatly limited due to radioactive contamination and the difficulty of single-cell detection, and was gradually replaced by the BrdU method based on antibody detection. The BrdU method has many steps and requires the use of BrdU antibody, which has many influencing factors and poor stability. EdU method is based on EdU incorporation and subsequent click reaction, without the use of antibodies, convenient operation and high detection sensitivity. It is a new method upgraded on the basis of BrdU method and will gradually replace BrdU method. EdU (5-ethynyl-2-deoxyuridine), is a thymidine analog, EdU can replace thymidine in the process of DNA synthesis to incorporate into new in synthetic DNA. On the other hand, the acetylene group on EdU can react with fluorescently labeled small molecule azide probes (such as FITC Azide, Fluor 488 Azide, Fluor 594 Azide, Fluor 647 Azide) through the catalysis of monovalent copper ions to form a stable triazole ring. This reaction is very rapid and is called the click reaction. Through the click reaction, the newly synthesized DNA is labeled with the corresponding fluorescent probe, so that the proliferating cells can be detected using the appropriate fluorescent detection equipment.
Product Categories/Family for EdU Cell Proliferation Flow Cytometry assay kit
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Product Notes
The EdU Cell Proliferation Flow Cytometry (Catalog #AAA178140) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EdU Cell Proliferation Flow Cytometry, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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