Standard Curve (Sample)
TUNEL Flow Cytometry Apoptosis Kit
One-step TUNEL Flow Cytometry Apoptosis Kit (Red, AF555)
Synonyms
TUNEL Flow Cytometry Apoptosis; N/A; One-step TUNEL Flow Cytometry Apoptosis Kit (Red, AF555); TUNEL Flow Cytometry Apoptosis kit
Form/Format
PBS buffer (with 1%BSA) (PH7.2 ~ 7.4)
Samples
Cell samples
Detection Instrument
Flow Cytometry
Dye Type
Fluor 555
Channel Set
PE/PE-TR
Detection Principle
When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.
Preparation and Storage
Store at -20 degree C, shading light
Estimated Expiration: 12 months
Shipping: Ice bag
Estimated Expiration: 12 months
Shipping: Ice bag
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TUNEL Flow Cytometry Apoptosis kit
Background: One-step TUNEL Flow Cytometry Apoptosis Kit applies a highly sensitive, fast and simple method to detect cell apoptosis. This kit can be used to detect apoptosis of suspension cells and adherent cells by flow cytometry.
Principle of the Assay: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. After fixation and permeabilization, the exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, and detected by flow cytometry.
Principle of the Assay: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. After fixation and permeabilization, the exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, and detected by flow cytometry.
Product Categories/Family for TUNEL Flow Cytometry Apoptosis kit
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Product Notes
The TUNEL Flow Cytometry Apoptosis (Catalog #AAA178150) is a Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TUNEL Flow Cytometry Apoptosis, Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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