Standard Curve (Sample)
Lipid Droplet Assay Kit
Lipid Droplet Isolation Kit
Synonyms
Lipid Droplet; N/A; Lipid Droplet Isolation Kit; Lipid Droplet assay kit
Preparation and Storage
Store the entire kit at 4 degree C
To avoid possible leakage store bottles upright.
To avoid possible leakage store bottles upright.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Lipid Droplet assay kit
Background/Introduction: Lipid droplets are organelles that are rich in lipids, contain a lipid rich core, and are surrounded by a phospholipid monolayer as well as outer lipid droplet associated proteins. Lipid droplets are commonly found in adipose tissue of animals, although they are found in all eukaryotes. Lipid droplets function to regulate the hydrolysis and storage of neutral lipids and also serve as storage for cholesterol and acyl-glycerols used to form and maintain cellular membranes. Beyond the function of lipid and cholesterol storage, lipid droplet organelles have been more recently associated with inflammatory responses, obesity, atherosclerosis, and cancer. Lipid droplets have been shown to protect against lipotoxicity in non-adipocytes by storing fatty acids as neutral triacylglycerol. Lipid droplets also aid in protein binding and degradation and have been demonstrated to be used by pathogens such as dengue virus and hepatitis C Virus. Lipid Droplet Isolation Kit isolates lipid droplets by simple gradient centrifugation, but circumvents the need for large sample sizes or ultracentrifugation. A lipid droplet source such as tissue or cultured cells is homogenized. A gradient is then created with the homogenate, and the material is centrifuged. The lipid droplets float to the top of the gradient and are recovered by carefully pipetting from the top of the gradient. Each kit provides sufficient reagents to isolate up to 50 preps based on a 50-100 mg tissue or cultured cell sample size.
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Product Notes
The Lipid Droplet (Catalog #AAA44629) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Lipid Droplet, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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