Standard Curve (Sample)
Mitochondrial Complex II Assay Kit
Mitochondrial Complex II Activity Assay Kit
Synonyms
Mitochondrial Complex II; N/A; Mitochondrial Complex II Activity Assay Kit; Mitochondrial Complex II assay kit
Specificity
48T(46 samples)/96T(94 samples)
Form/Format
Normal saline (0.9% NaCl), PBS(0.01 M, pH 7.4)
Samples
Animal tissue and cell samples
Detection Range
0.54-17.66 U/L
Sensitivity
0.54 U/L
Intra-assay Precision
Three rat heart tissue samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
Inter-assay Precision
Three rat heart tissue samples were assayed 20 times in duplicate by three operators to determine precision between assays.
Preparation and Storage
Store at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Mitochondrial Complex II assay kit
Principle of the Assay: Mitochondrial complex II, also known as succinate-coenzyme Q reductase, is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. It catalyzes the oxidation of succinic acid in the TCA cycle to fumaric acid, which converts the ubiquinone to a reduced form through the basic unit structures of the complex such as ironsulfur proteins. Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm.
Intended Uses: This kit can measure mitochondrial complex II activity in animal tissue and cell samples.
Intended Uses: This kit can measure mitochondrial complex II activity in animal tissue and cell samples.
Product Categories/Family for Mitochondrial Complex II assay kit
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Product Notes
The Mitochondrial Complex II (Catalog #AAA178095) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mitochondrial Complex II, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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