Standard Curve (Sample)
Potassium Ion Channel Assay Kit
Potassium Ion Channel Assay Kit
Synonyms
Potassium Ion Channel; N/A; Potassium Ion Channel Assay Kit; Elite Potassium Ion Channel Assay Kit; Potassium Ion Channel assay kit
Detection
FlexStation or Microplate reader
Kit Components
Component A: EliteTM Thallium Indicator Dye 1 vial (0.9 mg)
Component B: NYPR Solution, Probenecid Substitute 1 bottle (5 ml)
Component C: 10x KAB Buffer 1 bottle (10 ml)
Component B: NYPR Solution, Probenecid Substitute 1 bottle (5 ml)
Component C: 10x KAB Buffer 1 bottle (10 ml)
Preparation and Storage
Keep Component A at -20 degree C and avoid exposure to light. Component B at 4 degree C, and Component C at room temperature. All components are stable for 6 months after receipt if stored properly.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Potassium Ion Channel assay kit
Principle of the Assay: Potassium channels are a diverse and ubiquitous family of membrane proteins present in most cell types and control a wide variety of cell functions. Potassium (K+) channels play key role in regulating such processes as heart rate, hormone secretion, neurotransmitter release, electrolyte and water balance, and cell division/proliferation. Given their physiological importance, K+ channels have emerged as targets of drug discovery efforts for such indications as diabetes, epilepsy, pain, immunomodulation, arrhythmia, dementia, and others. The EliteTM Potassium Ion Channel kit is a no-wash cell based homogenous assay for high throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel activity, the assay is based on the activation of a proprietary fluorescent dye with a high affinity for thallium that reports potassium channel activity with a large fluorogenic signal which is proportional to the number of open potassium channels on the cell. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be read by a fluorescence microplate reader at Ex/Em = 488/525 nm (bottom read).
Product Categories/Family for Potassium Ion Channel assay kit
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Product Notes
The Potassium Ion Channel (Catalog #AAA62095) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Potassium Ion Channel, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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