Succinate Dehydrogenase (SDH) Activity Assay Kit | SDH assay kit
Succinate Dehydrogenase (SDH) Activity Assay Kit (Colorimetric)
Synonyms
Succinate Dehydrogenase (SDH) Activity; N/A; Succinate Dehydrogenase (SDH) Activity Assay Kit (Colorimetric); SDH assay kit
Samples
Animal and Plant Tissues, Cells and Bacteria
Usage Notes
deltaA of Test Tube should be between 0.01-0.3, the sample should be diluted further if deltaA of Test Tube >0.3.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract SDH from fresh samples in order to ensure the enzyme activity.
it is normal while there is fewer precipitates in Reagent III solution. Please filter if the precipitates interfer the result.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract SDH from fresh samples in order to ensure the enzyme activity.
it is normal while there is fewer precipitates in Reagent III solution. Please filter if the precipitates interfer the result.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Preparation and Storage
The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for SDH assay kit
Principle of the Assay: Succinate Dehydrogenase (SDH) is widely present in animals, plants, microorganisms and cultured cells. SDH is a marker enzyme of mitochondria and a membrane-bound enzyme located on the inner membrane of mitochondria. Also, it is one of the hubs to connect respiratory electron transfer and oxidative phosphorylation. In addition, SDH can provide electrons for the respiratory chain of various prokaryotic cell capacity. CheKine™ Micro Succinate Dehydrogenase (SDH) Assay Kit provides a simple method for detecting SDH activity in animal and plant tissues, cells and bacteria. The detection principle is based on that SDH can catalyze succinate to dehydrogenate and produce fumaric acid. The hydrogen can be transmited by Phenazine dimethyl ester sulphuric acid (PMS), thus reducing 2,6-Dichlorophenolindophenol (2,6-DCPIP) and 2,6-DCPIP has a special absorption peak at 605 nm. The reducing speed of 2,6-DCPIP can be determined by detecting the change of absorbance at 605 nm, then SDH activity in samples can be calculated.
Product Categories/Family for SDH assay kit
Similar Products
Product Notes
The SDH (Catalog #AAA332511) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Succinate Dehydrogenase (SDH) Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.