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TUNEL Assay Kit

TUNEL Assay Kit (HRP-DAB)

Applications
Apoptosis
Synonyms
TUNEL; N/A; TUNEL Assay Kit (HRP-DAB); TUNEL assay kit
Ordering
 
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For Research Use Only!
Applicable Applications for TUNEL assay kit
Apoptosis
Related Product Information for TUNEL assay kit
Background: The TUNEL In Situ Apoptosis Kit (HRP-DAB Method) has high sensitivity and easy operation. This kit is suitable for in situ apoptosis detection of tissue samples (paraffin sections, frozen sections) and cell samples (cell smears, slides), and the detection results can be observed by optical microscope.

Principle of the Assay: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with biotinlabeled dUTP, horseradish peroxidase (HRP)-labeled Streptavidin (Streptavidin-HRP) can be combined with biotin. So apoptotic cells can be observed by DAB reaction with optical microscope.

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Product Notes

The TUNEL (Catalog #AAA22132) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TUNEL can be used in a range of immunoassay formats including, but not limited to, Apoptosis. Researchers should empirically determine the suitability of the TUNEL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUNEL, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.