Application Data
(Fig. Fluorescence images using AAA Biotech TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min.)
TUNEL Detection Kit
TUNEL Apoptosis Detection Kit (Green Fluorescence)
Synonyms
TUNEL; N/A; TUNEL Apoptosis Detection Kit (Green Fluorescence); TUNEL detection kit
Preparation and Storage
Stable for at least 6 months at -20 degree C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components.
Application Data
(Fig. Fluorescence images using AAA Biotech TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min.)
Application Data
(Fig. Fluorescence images using AAA Biotech TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min.)
Related Product Information for TUNEL detection kit
Principle of the Assay: When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a fluorescein dUTP (FITC12-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), apoptosis cells can be detected by fluorescence microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis. The Enhanced Fluorescein Mix in the kit contains FITC-12-dUTP and fluorescence enhancement factor. The fluorescence enhancement factor can bind to FITC and enhance the stability amplifies of the signal, which makes the fluorescence brighter and has stronger anti-quenching ability
Background/Introduction: The TUNEL Apoptosis Assay Kit is a highly sensitive, rapid and simple method for cell apoptosis detection. The result can be detected by fluorescence microscopy. This kit is developed to detect tissue (Paraffin embedding, freezing and ultrathin section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection.
Background/Introduction: The TUNEL Apoptosis Assay Kit is a highly sensitive, rapid and simple method for cell apoptosis detection. The result can be detected by fluorescence microscopy. This kit is developed to detect tissue (Paraffin embedding, freezing and ultrathin section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection.
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Product Notes
The TUNEL (Catalog #AAA330133) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TUNEL, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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