Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Intended Uses
This AVPR1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine AVPR1A. This ELISA kit for research use only, not for therapeutic applications
!Intended Uses
This AVPR1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine AVPR1A. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for AVPR1A elisa kit
Principle of the Assay: AVPR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AVPR1A antibody and an AVPR1A-HRP conjugate. The assay sample and buffer are incubated together with AVPR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AVPR1A concentration since AVPR1A from samples and AVPR1A-HRP conjugate compete for the anti-AVPR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by AVPR1A from the sample, fewer sites are left to bind AVPR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AVPR1A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AVPR1A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,576 Da
NCBI Official Full Name
AVPR1A protein
NCBI Official Synonym Full Names
arginine vasopressin receptor 1A<
NCBI Official Symbol
AVPR1A
NCBI Protein Information
vasopressin V1a receptor
UniProt Protein Name
Vasopressin V1a receptor
UniProt Gene Name
AVPR1A
UniProt Entry Name
A2VDS9_BOVIN
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Product Notes
The Bovine AVPR1A avpr1a (Catalog #AAA94239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA94239 ELISA Kit recognizes Bovine AVPR1A. It is sometimes possible for the material contained within the vial of "Arginine Vasopressin Receptor 1A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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