FCM/FACS (Flow Cytometry)
(Immobilized Recombinant Human B2M at 2ug/mL (100uL/well) can bind Recombinant Human CD8 alpha with a linear range of 31.25-341 ng/mL.)
B2M/Beta-2-microglobulin Recombinant Protein | B2M recombinant protein
Recombinant Human B2M/Beta-2-microglobulin Protein
Gene Names
B2M; IMD43
Purity
>97% by SDS-PAGE.
Synonyms
B2M/Beta-2-microglobulin; N/A; Recombinant Human B2M/Beta-2-microglobulin Protein; B2M; IMD43; beta-2-microglobulin;IMD43; B2M recombinant protein
Host
HEK293 Cells
Purity/Purification
>97% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Sequence Length
119
Species
Human
Endotoxin
< 0.1 EU/ug of the protein by LAL method.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B2M at 2 ug/mL (100 uL/well) can bind Recombinant Human CD8 alpha with a linear range of 31.25-341 ng/mL.
Reconstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Tag
C-His
Preparation and Storage
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. Avoid repeated freeze/thaw cycles.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. Avoid repeated freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Immobilized Recombinant Human B2M at 2ug/mL (100uL/well) can bind Recombinant Human CD8 alpha with a linear range of 31.25-341 ng/mL.)
FCM/FACS (Flow Cytometry)
(Immobilized Recombinant Human B2M at 2ug/mL (100uL/well) can bind Recombinant Human CD8 alpha with a linear range of 31.25-341 ng/mL.)
SDS-PAGE
(Recombinant Human B2M/Beta-2-microglobulin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 13 kDa.)
SDS-PAGE
(Recombinant Human B2M/Beta-2-microglobulin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 13 kDa.)
Related Product Information for B2M recombinant protein
Description: Recombinant Human B2M/Beta-2-microglobulin Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ile21-Met119) of human B2M/Beta-2-microglobulin (Accession #NP_004039.1) fused with a 6xHis tag at the C-terminus.
Background: This protein is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Background: This protein is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Product Categories/Family for B2M recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Official Synonym Symbols
IMD43
NCBI Protein Information
beta-2-microglobulin
UniProt Protein Name
Beta-2-microglobulin
UniProt Gene Name
B2M
UniProt Entry Name
B2MG_HUMAN
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Product Notes
The B2M b2m (Catalog #AAA281426) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "B2M/Beta-2-microglobulin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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