IL-5 active protein

Recombinant mouse IL-5 Protein

Cat. Num. AAA48401

• Gene Names: Il5; Il-5
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: IL-5; N/A; Recombinant mouse IL-5 Protein; iinterleukin 5; Il; Il-5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF; B-cell differentiation factor I; EDF; IL5; TRFB cell differentiation factor I; IL-5 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 21-133aa
• Sequence: MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG

• Applicable Applications for IL-5 active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Met 21
• Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range <= 1ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Mouse IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for IL-5 active protein

IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. This interleukin produced by type-2 T helper cells and mast cells. It related hematopoietic cytokines that are important for allergic inflammation.. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Recombinant mouse IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-5 active protein

Immunology; Proteins

References

Shen HH., et al, (2003) J. Immunol. 170 (6): 3296-3305. Sanderson CJ., et al, (1992) Blood. 79 (12): 3101-3109.

NCBI and Uniprot Product Information

• NCBI GI #: 6754336
• NCBI GeneID: 16191
• NCBI Accession #: NP_034688
• NCBI GenBank Nucleotide #: NM_010558.1
• UniProt Accession #: P04401
• Molecular Weight: 26.0 kDa (predicted detection band MW)
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: Il5
• NCBI Official Synonym Symbols: Il-5
• NCBI Protein Information: interleukin-5
• UniProt Protein Name: Interleukin-5
• UniProt Gene Name: Il5
• UniProt Synonym Gene Names: Il-5; IL-5; BCGF-II; TRF
• UniProt Entry Name: IL5_MOUSE