Human IL-5 ELISA Kit | IL-5 elisa kit

Human IL-5 PicoKine Fast ELISA Kit

Cat. Num. AAA125320

• Gene Names: IL5; EDF; TRF; IL-5
• Reactivity: Human
• Synonyms: IL-5; N/A; Human IL-5 PicoKine Fast ELISA Kit; Human IL-5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF; IL5; IL-5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL5
• Sequence Length: 134
• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and urine
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: <2pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for IL-5 elisa kit

Principle of the Assay: The Picokine™ Human IL5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL5 with a 96-well strip plate that is pre-coated with antibody specific for IL5. The detection antibody is a biotinylated antibody specific for IL5. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IL5 with immunogen: Expression system for standard: sf21; Immunogen sequence: I20-S134. The kit is analytically validated with ready to use reagents. To measure Human IL5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human IL5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL5 in the sample.!!Background/Introduction: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NP_000870.1
• UniProt Accession #: P05113
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5
• UniProt Protein Name: Interleukin-5
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN