NRG1/HRG1 active protein

Recombinant human NRG1/HRG1 protein

Cat. Num. AAA48387

• Gene Names: NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: NRG1/HRG1; N/A; Recombinant human NRG1/HRG1 protein; NRG1; ARIA; GGF2; GGF; GGFglial growth factor; HGL; HGLneu differentiation factor; HRG; HRG1; HRGA; NRG-IT2; NRG1-IT2; HRG1-alpha; HRG1-beta 1; HRGAneuregulin 1 type IV beta 1a; Heregulin-1; MST131; MSTP131; NDFheregulin; alpha (45kD; ERBB2 p185-activator); Neuregulin-1; Neuregulin1; SMDF; neuregulin 1; neuregulin 1 type IV beta 3; pro-NRG1; pro-neuregulin-1; membrane-bound isoform; sensory and motor neuron derived factor; NRG1/HRG1 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-241aa
• Sequence: SGKKPES AAGSQSPALP PRLKEMKSQE SAAGSKLVLR CETSSEYSSL RFKWFKNGNE LNRKNKPQNI KIQKKPGKSE LRINKASLAD SGEYMCKVIS KLGNDSASAN ITIVESNEII TGMPASTEGA YVSSESPIRI SVSTEGANTS SSTSTSTTGT SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYSTSTPF LSLPE

• Applicable Applications for NRG1/HRG1 active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range <= 15ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human Proinsulin in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for NRG1/HRG1 active protein

Neuregulin-1 (NRG1), also known as ARIA, is one of four proteins in the neuregulin family that act on the EGFRfamily of receptors. It has multiple isoforms that aregenerated by usage of different promoters and alternative splicing of a single gene. This protein regulates multiple nervous system functions including axon guidance, synapse formation and plasticity, glial cell development, and axon myelination. In the heart, it regulates organ morphogenesis and contractility and also plays a cardioprotective role following tissue injury. NRG1 is essential for the development and function of multiple organ systems, and itsdysregulation has been linked to diseases such as cancer and schizophrenia. Recombinant human NRG1,fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatographytechniques.

Product Categories/Family for NRG1/HRG1 active protein

Neuroscience; Proteins

References

Tan W, et al. (2007). J Biol Chem. 282:24343-24351. Nicodemus KK, et al. (2010). Arch Gen Psychiatry. 67:991-1001.

NCBI and Uniprot Product Information

• NCBI GI #: 116006959
• NCBI GeneID: 3084
• NCBI Accession #: NP_039252
• NCBI GenBank Nucleotide #: NM_013958.3
• UniProt Accession #: Q02297; O14667; P98202; Q02298; Q02299; Q07110; Q07111; A5YAK4; A5YAK5; A8K1L2; B7Z4Z3; E9PHH4
• Molecular Weight: 46,226 Da
• NCBI Official Full Name: pro-neuregulin-1, membrane-bound isoform isoform HRG-beta3
• NCBI Official Synonym Full Names: neuregulin 1
• NCBI Official Symbol: NRG1
• NCBI Official Synonym Symbols: GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2
• NCBI Protein Information: pro-neuregulin-1, membrane-bound isoform; glial growth factor; heregulin, alpha (45kD, ERBB2 p185-activator); neu differentiation factor; pro-NRG1; sensory and motor neuron derived factor
• UniProt Protein Name: Pro-neuregulin-1, membrane-bound isoform
• UniProt Gene Name: NRG1
• UniProt Synonym Gene Names: GGF; HGL; HRGA; NDF; SMDF; Pro-NRG1; ARIA; HRG
• UniProt Entry Name: NRG1_HUMAN