Bioactivity
(HTRF assay for RPS6KA4/RSK4 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KA4/RSK4 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)
RPS6KA4/RSK4 active protein
Recombinant RPS6KA4/RSK4 protein
Purity
>50%
Synonyms
RPS6KA4/RSK4; N/A; Recombinant RPS6KA4/RSK4 protein; Robosomal Protein S6 Kinase A4; RPS6KA4/RSK4 active protein
Host
Baculovirus
Purity/Purification
>50%
Form/Format
Recombinant RPS6KA4/RSK4 protein is supplied in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Protein Details
Recombinant RPS6KA4/RSK4 protein was expressed in baculovirus expression system as the full length protein (accession number NP_003933.1) with a N-terminal DYKDDDDK tag. The molecular weight of the protein is 86.9 kDa.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Bioactivity
(HTRF assay for RPS6KA4/RSK4 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KA4/RSK4 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)
Bioactivity
(HTRF assay for RPS6KA4/RSK4 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KA4/RSK4 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)
Bioactivity
(HTRF assay for RPS6KA4/RSK4 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KA4/RSK4 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)
Bioactivity
(HTRF assay for RPS6KA4/RSK4 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KA4/RSK4 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)
SDS-PAGE
(Recombinant PRDM4 protein 10% SDS-PAGE Coomassie staining MW: 86.9 kDa Purity: >= 50%)
SDS-PAGE
(Recombinant PRDM4 protein 10% SDS-PAGE Coomassie staining MW: 86.9 kDa Purity: >= 50%)
Related Product Information for RPS6KA4/RSK4 active protein
RPS6KA4 (Ribosomal Protein S6 Kinase A4) is a serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. RPS6KA4 phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin and phosphorylates 'Ser-10’ of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN.
Product Categories/Family for RPS6KA4/RSK4 active protein
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Product Notes
The RPS6KA4/RSK4 (Catalog #AAA60317) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPS6KA4/RSK4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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