AcmNPV Envelope glyco Cell Lysate | AcMNPV-GP64 cell lysate
AcmNPV Envelope glyco Insect Cell Lysate (WB positive control)
Gene Names
Ac-gp64; AcOrf-128
Applications
Western Blot
Synonyms
AcmNPV Envelope glyco; N/A; AcmNPV Envelope glyco Insect Cell Lysate (WB positive control); AcMNPV gp64 Overexpression Lysate; AcMNPV-GP64 cell lysate
Host
Baculovirus-Insect Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
472aa
Sequence
Met1-Thr481
Applicable Applications for AcMNPV-GP64 cell lysate
WB (Western Blot)
Species
AcMNPV
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4°C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80
2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4°C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80
Predicted N Terminal
Ala 21
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue after protein purification
Sequence Information
A DNA sequence encoding the autographa californica nucleopolyhedrovirus (AcNPV) (strain E2) gp64 (NP_054158.1) (Met1-Thr481) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4°C. After re-dissolution, aliquot and store at -80°C.
Related Product Information for AcMNPV-GP64 cell lysate
Description: Baculovirus-Insect Cell Lysate that AcmNPV Envelope glyco transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Molecule Mass: The recombinant autographa californica nucleopolyhedrovirus (AcNPV)(strain E2) gp64 consists of 472 amino acids and predicts a molecular mass of 54.2 kDa
NCBI Official Full Name
major budded virus envelope glycoprotein
NCBI Official Symbol
Ac-gp64
NCBI Official Synonym Symbols
AcOrf-128
NCBI Protein Information
gp67
UniProt Protein Name
Major envelope glycoprotein
UniProt Gene Name
GP64
UniProt Synonym Gene Names
GP67
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Product Notes
The AcMNPV-GP64 gp64 (Catalog #AAA257380) is a Cell Lysate produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 472aa. AAA Biotech's AcmNPV Envelope glyco can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the AcMNPV-GP64 gp64 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr48 1. It is sometimes possible for the material contained within the vial of "AcmNPV Envelope glyco, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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