COVID 19 Spike S1+S2 Coronavirus Active Protein | COVID-19 active protein

SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)

Cat. Num. AAA268887

• Purity: >90 % as determined by SDS-PAG
• Synonyms: COVID 19 Spike S1+S2 Coronavirus; N/A; SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1+S2; COVID-19 active protein

• Host: Baculovirus-Insect Cells; Source: 2019nCoV
• Purity/Purification: >90 % as determined by SDS-PAG
• Form/Format: Liquid or lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

• Reconstitution: Detailed reconstitution instructions are sent along with the products
• Predicted N terminal: Val 16
• Biological Activity: Immobilized Spike Protein (S1+S2 ECD, His tag) (ZD2019-P1) at156.5-10000ng/mL (100uL/well) can bind 10ug/ml of Human ACE2, Fc Tag
• Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2ECD) (YP_009724390.1) (Val16-Pro1213) was expressed with a polyhistidine tag at the C-terminus
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)consists of 1209 amino acids and predicts a molecular mass of 134.36 kDa.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Samples are stable for twelve months from date of receipt at -20 degree C to -80 degree C.; Store it under sterile conditions at -20 degree C to -80 degree C upon receiving.; Recommend to aliquot the protein intosmaller quantities for optimal storage. Avoid repeated freeze-thaw cycles

Application Data

Application Data

Related Product Information for COVID-19 active protein

Description:
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptorson the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidylpeptidase-4; APN aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesionmolecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both hostspecificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologousproteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is knownto be essential in the binding of the virus to the host cell at the of the infection process. It's been reportedthat 2019-nCoV can infect thehuman respiratory epithelial cells through interaction with the human ACE2receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2.S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cellsurface receptor. S2 contains basic elements needed for the membrane fusion.

References

References:
1.Shen S, et al. (2007) Expression, glycosylation, and modification of thespike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
2.Du L,et al. (2009) The spike protein of SARS-CoV--a target for vaccine andtherapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
3.Xiao X, et al.(2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.