SIRP gamma/CD172g Active Protein | SIRPgamma/CD172g active protein

Recombinant human SIRP gamma/CD172g protein

Cat. Num. AAA48402

• Gene Names: SIRPG; CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: SIRP gamma/CD172g; N/A; Recombinant human SIRP gamma/CD172g protein; Signal-regulatory protein gamma isoform 1; SIRPG; bA77C3.1; CD172g; SIRP-B2; SIRPB2; SIRPgamma; SIRPgamma/CD172g active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 20% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 29-360aa
• Sequence: EEELQMI QPEKLLLVTV GKTATLHCTV TSLLPVGPVL WFRGVGPGRE LIYNQKEGHF PRVTTVSDLT KRNNMDFSIR ISSITPADVG TYYCVKFRKG SPENVEFKSG PGTEMALGAK PSAPVVLGPA ARTTPEHTVS FTCESHGFSP RDITLKWFKN GNELSDFQTN VDPTGQSVAY SIRSTARVVL DPWDVRSQVI CEVAHVTLQG DPLRGTANLS EAIRVPPTLE VTQQPMRVGN QVNVTCQVRK FYPQSLQLTW SENGNVCQRE TASTLTENKD GTYNWTSWFL VNISDQRDDV VLTCQVKHDG QLAVSKRLAL EVTVHQKDQS SDATP

• Applicable Applications for SIRPgamma/CD172g active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured by its binding ability in a functional ELISA with Human CD47. The ED50 range <= 1ug/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human CD47 (CAT# ATGP4015) is coated at 1 ug/ml (100 ul/well) can bind Human SIRP gamma/CD172g. The ED range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for SIRPgamma/CD172g active protein

SIRPG, also known as signal-regulatory protein gamma isoform 1, is a member of a closely related family of three cell surface receptors implicated in modulating immune/inflammatory responses. It is the only SIRP known to be expressed on T cells, CD56 bright NK cells and activated NK cells. Expression of SIRPG on T cells suggests a role as an accessory protein interacting with CD47?expressing antigen presenting cells. Engagement of CD47 by SIRPG was shown to induce apoptosis on T-cell and monocyte cell lines. Recombinant human SIRPG, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for SIRPgamma/CD172g active protein

Immunology; Adhesion & Movement; Proteins

References

Nettleship JE., et al. (2013) BMC Struct Biol. 13:13. Brooke G., et al. (2004) J Immunol. 173:2562-2570.

NCBI and Uniprot Product Information

• NCBI GI #: 94538335
• NCBI GeneID: 55423
• NCBI Accession #: NP_061026.2
• NCBI GenBank Nucleotide #: NM_018556.3
• UniProt Accession #: Q9P1W8; Q5D051; Q5JV25; Q5MKL4; Q8WWA5; Q9NQK8; B1AKP6
• Molecular Weight: 29,967 Da
• NCBI Official Full Name: signal-regulatory protein gamma isoform 1
• NCBI Official Synonym Full Names: signal regulatory protein gamma
• NCBI Official Symbol: SIRPG
• NCBI Official Synonym Symbols: CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• NCBI Protein Information: signal-regulatory protein gamma
• UniProt Protein Name: Signal-regulatory protein gamma
• UniProt Gene Name: SIRPG
• UniProt Synonym Gene Names: SIRPB2; SIRP-gamma; SIRP-b2; SIRP-beta-2
• UniProt Entry Name: SIRPG_HUMAN