Mouse B-cell CLL/lymphoma 2 ELISA Kit | BCL2 elisa kit

Mouse B-cell leukemia/lymphoma 2, Bcl-2 ELISA Kit

Cat. Num. AAA79452

• Gene Names: Bcl2; Bcl-2; AW986256; C430015F12Rik; D630044D05Rik; D830018M01Rik
• Reactivity: Mouse
• Synonyms: B-cell CLL/lymphoma 2; N/A; Mouse B-cell leukemia/lymphoma 2, Bcl-2 ELISA Kit; Mouse B-cell leukemia/lymphoma 2; Bcl-2 ELISA Kit; Bcl-2; B-cell lymphoma protein 2; OTTHUMP00000163680; BCL2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse BCL2. No significant cross-reactivity or interference between Mouse BCL2 and analogues was observed.
• Sequence Length: 236
• Samples: Serum, plasma
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 3.9 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for BCL2 elisa kit

Introduction: Bcl-2 is the prototype for a family of mammalian genes and the proteins they produce. They govern mitochondrial outer membrane permeabilization (MOMP) and can be either pro-apoptotic (Bax, BAD, Bak and Bok among others) or anti-apoptotic (including Bcl-2 proper, Bcl-xL, and Bcl-w, among an assortment of others). There are a total of 25 genes in the Bcl-2 family known to date. The members of the Bcl-2 family share one or more of the four characteristic domains of homology entitled the Bcl-2 homology (BH) domains (named BH1, BH2, BH3 and BH4). The BH domains are known to be crucial for function, as deletion of these domains via molecular cloning affects survival/apoptosis rates. The anti-apoptotic Bcl-2 proteins, such as Bcl-2 and Bcl-xL, conserve all four BH domains. The BH domains also serve to subdivide the pro-apoptotic Bcl-2 proteins into those with several BH domains (e.g. Bax and Bak) or those proteins that have only the BH3 domain (e.g. Bid, Bim and Bad). The Bcl-2 family has a general structure that consists of a hydrophobic helix surrounded by amphipathic helices. Many members of the family have transmembrane domains. The site of action for the Bcl-2 family is mostly on the outer mitochondrial membrane. Within the mitochondria are apoptogenic factors that if released activate the executioners of apoptosis, the caspases. Depending on their function, once activated, Bcl-2 proteins either promote the release of these factors, or keep them sequestered in the mitochondria. Whereas the activated pro-apoptotic Bak and/or Bax would form MAC and mediate the release of cytochrome c, the anti-apoptotic Bcl-2 would block it, possibly through inhibition of Bax and/or Bak. There are a number of theories concerning how the Bcl-2 gene family exert their pro- or anti-apoptotic effect. An important one states that this is achieved by activation or inactivation of an inner mitochondrial permeability transition pore, which is involved in the regulation of matrix Ca2+, pH, and voltage. It is also thought that some Bcl-2 family proteins can induce or inhibit the release of cytochrome c in to the cytosol which, once there, activates caspase-9 and caspase-3, leading to apoptosis.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bcl-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Bcl-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Bcl-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 133892547
• NCBI GeneID: 12043
• NCBI Accession #: NP_033871.2
• NCBI GenBank Nucleotide #: NM_009741.4
• UniProt Accession #: P10417; P10418; Q4VBF6
• Molecular Weight: 26,407 Da
• NCBI Official Full Name: apoptosis regulator Bcl-2 isoform 1
• NCBI Official Synonym Full Names: B cell leukemia/lymphoma 2
• NCBI Official Symbol: Bcl2
• NCBI Official Synonym Symbols: Bcl-2; AW986256; C430015F12Rik; D630044D05Rik; D830018M01Rik
• NCBI Protein Information: apoptosis regulator Bcl-2; B-cell leukemia/lymphoma 2
• UniProt Protein Name: Apoptosis regulator Bcl-2
• UniProt Gene Name: Bcl2
• UniProt Synonym Gene Names: Bcl-2
• UniProt Entry Name: BCL2_MOUSE