Standard Curve (Sample)
(Typical Standard Curve for Bcl3 ELISA (Sandwich))
Rat B-Cell CLL/Lymphoma 3 ELISA Kit | Bcl3 elisa kit
Rat B-Cell CLL/Lymphoma 3 (Bcl3) (Sandwich ELISA) ELISA Kit
Reactivity
Rat
Synonyms
B-Cell CLL/Lymphoma 3; N/A; Rat B-Cell CLL/Lymphoma 3 (Bcl3) (Sandwich ELISA) ELISA Kit; BCL3; BCL4; B-Cell Lymphoma 3-Encoded Protein; Chronic Lymphatic Leukemia Protein; Proto-oncogene BCL3; ELISA Kit for B-Cell CLL/Lymphoma 3 (Bcl3); Bcl3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 3 (Bcl3).
No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 3 (Bcl3) and analogues was observed. Reactive with Rat Bcl3 / B-Cell CLL/Lymphoma 3
No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 3 (Bcl3) and analogues was observed. Reactive with Rat Bcl3 / B-Cell CLL/Lymphoma 3
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.054ng/mL
Detection Method
Colormetric
Assay Precision
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Preparation and Storage
4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year
Standard Curve (Sample)
(Typical Standard Curve for Bcl3 ELISA (Sandwich))
Standard Curve (Sample)
(Typical Standard Curve for Bcl3 ELISA (Sandwich))
Related Product Information for Bcl3 elisa kit
Principle of the Assay||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 3 (Bcl3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 3 (Bcl3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 3 (Bcl3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 3 (Bcl3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for Bcl3 elisa kit
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Rat Bcl3 bcl3 (Catalog #AAA189968) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189968 ELISA Kit recognizes Rat Bcl3. It is sometimes possible for the material contained within the vial of "B-Cell CLL/Lymphoma 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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