Application Data
BCR-RET recombinant protein
BCR-RET Mutant, Active
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Applications
Kinase Assay
Synonyms
BCR-RET; N/A; BCR-RET Mutant, Active; BCR-RET recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
1-426 (BCR) - 713-end (RET)
Sequence Length
4686
Applicable Applications for BCR-RET recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for BCR-RET recombinant protein
Description: Recombinant human BCR-RET, the fusion protein [BCR (1-426)-RET (713-end)], was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: BCR-RET is a chimaeric fusion of BCR and RET genes generated by a balanced translocation of genes from t (10;22) (q11;q11) and this leads to aberrant activation of RET activity which can transform hematopoietic cells and skew the hematopoietic differentiation program towards the monocytic/macrophage lineage (1). The BCR-RET fusion is frequently found in chronic myelomonocytic leukemia (CMML) cases. The RET fusion genes seem to constitutively mimic the same signaling pathway as RAS mutations frequently involved in CMML.
Scientific Background: BCR-RET is a chimaeric fusion of BCR and RET genes generated by a balanced translocation of genes from t (10;22) (q11;q11) and this leads to aberrant activation of RET activity which can transform hematopoietic cells and skew the hematopoietic differentiation program towards the monocytic/macrophage lineage (1). The BCR-RET fusion is frequently found in chronic myelomonocytic leukemia (CMML) cases. The RET fusion genes seem to constitutively mimic the same signaling pathway as RAS mutations frequently involved in CMML.
Product Categories/Family for BCR-RET recombinant protein
References
1. Ballerini, P. et al: RET fusion genes are associated with chronic myelomonocytic leukemia and enhance monocytic differentiation. Leukemia. 2012 Nov;26 (11):2384-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
140 kDa
NCBI Official Full Name
Homo sapiens breakpoint cluster region, mRNA
NCBI Official Synonym Full Names
BCR, RhoGEF and GTPase activating protein
NCBI Official Symbol
BCR
NCBI Official Synonym Symbols
ALL; CML; PHL; BCR1; D22S11; D22S662
NCBI Protein Information
breakpoint cluster region protein
UniProt Protein Name
Breakpoint cluster region protein
UniProt Gene Name
BCR
UniProt Synonym Gene Names
BCR1; D22S11
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Product Notes
The BCR-RET bcr (Catalog #AAA73112) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-426 (BCR) - 713-end (RET). AAA Biotech's BCR-RET can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the BCR-RET bcr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCR-RET, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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